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Plant and Cell Physiology Advance Access published online on November 23, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci233
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received November 4, 2005
Accepted November 18, 2005

Rapid Paper

EUI1, Encoding a Putative Cytochrome P450 Monooxygenase, Regulates the Internodes Elongation by Modulating GA Responses in Rice

Anding Luo 1 6, Qian Qian 2 6, Hengfu Yin 3, Xiaoqiang Liu 1, Changxi Yin 4, Ying Lan 1, Jiuyou Tang 1, Zuoshun Tang 1, Shouyun Cao 1, Xiujie Wang 1, Kai Xia 4, Xiangdong Fu 5, Da Luo 3, and Chengcai Chu 1 *

1 National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
2 China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, China
3 National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Shanghai 200032, China
4 College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
5 The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

* To whom correspondence should be addressed.
Chengcai Chu, E-mail: ccchu{at}genetics.ac.cn


  Abstract

Elongation of rice internodes is one of the most important agronomic traits, which determines the plant height and underlies the grain yield. It has been shown that the elongation of internodes is under genetic control and various factors are implicated in the process. Here, we report a detailed characterization of an elongated uppermost internode1 (eui1) mutant, which has been used in hybrid rice breeding. In the eui1-2 mutant, the cell lengths in the uppermost internodes are significantly longer than that of wild type and thus give rise to the elongated uppermost internode. It was found that the level of active gibberellic acids (GAs) was elevated in the mutant, whereas its growth in response to GA was similar to that of wild type, suggesting the higher level accumulation of GA in the eui1 causes the abnormal elongation of the uppermost internode. Consistently, the expression levels of several genes, which encode gibberellin biosynthesis enzymes, were altered. We cloned the EUI1 gene by map-based cloning, which encodes a putative cytochrome P450 monooxygenase, and found that EUI1 was weakly expressed in most tissues, but preferentially in young panicles. To confirm its function, transgenic experiments with different constructs of EUI1 were conducted. Overexpression of EUI1 gave rise to the GA-deficient like phenotypes, which could be partially reversed by the supplement of GA. Furthermore, apart from the alteration of expression levels of the GA biosynthesis genes, accumulation of SLR1 protein was found in the overexpression transgenic plants, indicating expression level of EUI1 is implicated in both GA-mediated SLR1 destruction and a feedback regulation in GA biosynthesis. Therefore, we proposed that EUI1 plays a negative role in GA-mediated regulation of cell elongation in the uppermost internode of rice.

Keywords: Cytochrome P450 monooxygenase; Elongated uppermost internode 1; Gibberellin; Internode elongation; Rice.

6 These authors contributed equally.


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