Plant and Cell Physiology Advance Access published online on November 12, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci230
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1 Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, 305-8686 Japan
* To whom correspondence should be addressed. The transcription factors DREB1s/CBFs specifically interact with the DRE/CRT cis-acting element and control the expression of many stress-inducible genes in Arabidopsis. The genes for DREB1 orthologues, OsDREB1A and OsDREB1B from rice, are induced by cold stress, and overexpression of DREB1 or OsDREB1 induced strong expression of stress-responsive genes in transgenic Arabidopsis plants, resulting in increased tolerance to high-salt and freezing stresses. In this study we generated transgenic rice plants overexpressing the OsDREB1 or DREB1 genes. These transgenic rice plants showed not only growth retardation under normal growth conditions but also improved tolerance to drought, high-salt and low-temperature stresses like the transgenic Arabidopsis plants overexpressing OsDREB1 or DREB1. We also detected elevated contents of osmoprotectants such as free proline and various soluble sugars in the transgenic rice as in the transgenic Arabidopsis plants. We identified target stress-inducible genes of OsDREB1A in the transgenic rice using microarray and RNA gel blot analyses. These genes encode proteins that are thought to function in stress tolerance in the plants. These results indicate that the DREB1/CBF cold-responsive pathway is conserved in rice and the DREB1-type genes are quite useful for improvement of stress tolerance to environmental stresses in various kinds of transgenic plants including rice.
Received May 30, 2005
Accepted November 7, 2005
Regular Paper
Functional Analysis of Rice DREB1/CBF-Type Transcription Factors Involved in Cold-Responsive Gene Expression in Transgenic Rice
Yusuke Ito 1,
Koji Katsura 1,
Kyonoshin Maruyama 1,
Teruaki Taji 2,
Masatomo Kobayashi 3,
Motoaki Seki 4,
Kazuo Shinozaki 5,
and
Kazuko Yamaguchi-Shinozaki 6 *
2 Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, Tsukuba, Ibaraki, 305-0074 Japan; Present address: Department of Bio-Science, Tokyo University of Agriculture, Setagaya, Tokyo, 156-8502 Japan
3 Experimental Plant Division, BioResource Center, RIKEN Tsukuba Institute, Tsukuba, Ibaraki, 305-0074 Japan
4 Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, Tsukuba, Ibaraki, 305-0074 Japan
5 Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, Tsukuba, Ibaraki, 305-0074 Japan; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Kawaguchi, Saitama, 332-0012 Japan; Present address: RIKEN Plant Science Center, Tsurumi, Yokohama, 203-0045 Japan
6 Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, 305-8686 Japan; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, Kawaguchi, Saitama, 332-0012 Japan; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657 Japan
Kazuko Yamaguchi-Shinozaki, E-mail: kazukoys{at}jircas.affrc.go.jp
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