Skip Navigation



Plant and Cell Physiology Advance Access published online on October 25, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci218
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
47/1/14    most recent
pci218v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Elorza, A.
Right arrow Articles by Jordana, X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Elorza, A.
Right arrow Articles by Jordana, X.
Agricola
Right arrow Articles by Elorza, A.
Right arrow Articles by Jordana, X.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received August 18, 2005
Accepted October 16, 2005

Regular Paper

A Nuclear Gene for the Iron-Sulfur Subunit of Mitochondrial Complex II Is Specifically Expressed During Arabidopsis Seed Development and Germination

Alvaro Elorza 1, Hannetz Roschzttardtz 2, Isabel Gómez 2, Armand Mouras 3, Loreto Holuigue 2, Alejandro Araya 4, and Xavier Jordana 2*

1 Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, P. Universidad Católica de Chile, Casilla 114-D, Santiago, Chile; UMR 0619-Physiologie et Biotechnologie Végétales, Institut National de la Recherche Agronomique et Université Victor Segalen-Bordeaux II, IBVM CR-INRA Bordeaux, B.P. 81, 33883 Villenave d'Ornon Cedex, France; Laboratoire de Réplication et Expression des Gènes Eucaryotes et Rétroviraux, UMR 5097, CNRS et Université Victor Segalen-Bordeaux II, 33076 Bordeaux-Cedex, France
2 Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, P. Universidad Católica de Chile, Casilla 114-D, Santiago, Chile
3 UMR 0619-Physiologie et Biotechnologie Végétales, Institut National de la Recherche Agronomique et Université Victor Segalen-Bordeaux II, IBVM CR-INRA Bordeaux, B.P. 81, 33883 Villenave d'Ornon Cedex, France
4 Laboratoire de Réplication et Expression des Gènes Eucaryotes et Rétroviraux, UMR 5097, CNRS et Université Victor Segalen-Bordeaux II, 33076 Bordeaux-Cedex, France

* To whom correspondence should be addressed.
Xavier Jordana, E-mail: xjordana{at}bio.puc.cl


  Abstract

Three nuclear genes, SDH2-1, SDH2-2 and SDH2-3, encode the essential iron-sulfur subunit of mitochondrial complex II in Arabidopsis thaliana. SDH2-1 and SDH2-2 likely arose via a recent duplication event and we reported that both are expressed in all organs from adult plants. In contrast, transcripts from SDH2-3 were not detected. Here we present data demonstrating that SDH2-3 is specifically expressed during seed development. SDH2-3 transcripts appear during seed maturation, persist through desiccation, are abundant in dry seeds, and markedly decline during germination. Analysis of transgenic Arabidopsis plants carrying the SDH2-3 promoter fused to the {beta}-glucuronidase reporter gene shows that the SDH2-3 promoter is activated in the embryo during maturation, from the bent-cotyledon stage. {beta}-glucuronidase expression correlates with the appearance of endogenous SDH2-3 transcripts, suggesting that control of this nuclear gene is achieved through transcriptional regulation. Furthermore, progressive deletions of this promoter identified a 159 bp region (-223 to -65) important for SDH2-3 transcriptional activation in seeds. Interestingly, the SDH2-3 promoter remains active in embryonic tissues during germination and post-germinative growth, and is turned off in vegetative tissues (true leaves). In contrast to SDH2-3 transcripts, SDH2-1 and SDH2-2 transcripts are barely detected in dry seeds and increase during germination and post-germinative growth. The opposite expression patterns of SDH2 nuclear genes strongly suggest that during germination the embryo specific SDH2-3 is replaced by SDH2-1 or SDH2-2 in mitochondrial complex II.

Keywords: Arabidopsis thaliana; iron-sulfur subunit; plant mitochondria; seed-specific expression; succinate dehydrogenase; transcriptional regulation.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
H. Roschzttardtz, I. Fuentes, M. Vasquez, C. Corvalan, G. Leon, I. Gomez, A. Araya, L. Holuigue, J. Vicente-Carbajosa, and X. Jordana
A Nuclear Gene Encoding the Iron-Sulfur Subunit of Mitochondrial Complex II Is Regulated by B3 Domain Transcription Factors during Seed Development in Arabidopsis
Plant Physiology, May 1, 2009; 150(1): 84 - 95.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Welchen, I. L. Viola, H. J. Kim, L. P. Prendes, R. N. Comelli, J. C. Hong, and D. H. Gonzalez
A segment containing a G-box and an ACGT motif confers differential expression characteristics and responses to the Arabidopsis Cytc-2 gene, encoding an isoform of cytochrome c
J. Exp. Bot., March 1, 2009; 60(3): 829 - 845.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Leon, L. Holuigue, and X. Jordana
Mitochondrial Complex II Is Essential for Gametophyte Development in Arabidopsis
Plant Physiology, April 1, 2007; 143(4): 1534 - 1546.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.