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Plant and Cell Physiology Advance Access published online on October 13, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci209
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received April 18, 2005
Accepted September 27, 2005

Regular Paper

Carbonic Anhydrase Activity of the Photosystem II OEC-33 Protein from Pea

Yih-Kuang Lu 1, Steven M. Theg 2, and Alan J. Stemler 2*

1 Dept of Chemistry and Biochemistry, Arizona State University Tempe, AZ 85287 USA
2 Section of Plant Biology, University of California, Davis, CA 95616 USA

* To whom correspondence should be addressed.
Alan J. Stemler, E-mail: ajstemler{at}ucdavis.edu


  Abstract

The purpose of this study was to identify the location of one of the two sources of carbonic anhydrase activity associated with the photosystem II (PSII) complex in chloroplast membranes. We tested the hypothesis that the extrinsic 33kD protein, OEC33, associated with the oxygen-evolving complex, is one source of CA activity. We found that precursor OEC33 expressed in Escherichia coli exhibits CA activity, but not the expressed precursor of the OEC24 or OEC17. The CA activity of the OEC 33 remained after treatment at 90°C for 15 min. Additional biochemical evidence supports the hypothesis. Only those wash treatments that remove the OEC33 from PSII also remove CA activity. Both immunoblot and CA activity show that the CA tracks the OEC33, in parallel, when PSII undergoes washing at different CaCl2 concentrations. The OEC33 protein purified by HiTrap Q anion exchange chromatography has CA activity that is inhibited by an antibody against the OEC33. PSII membranes washed with 1 M CaCl2 to remove the OEC33 can be reconstituted with either extracted, purified, OEC33 or with the E. coli-expressed precursor OEC33. Reconstitution partially restores both oxygen evolution and CA activity. For maximal CA activity, the OEC33 requires manganese as a cofactor.

Keywords: Bicarbonate; Carbonic anhydrase; Manganese Stabilizing Protein; OEC33; Pisum sativum; Photosystem II.
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[Abstract] [Full Text] [PDF]


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