Skip Navigation



Plant and Cell Physiology Advance Access published online on September 17, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci194
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
46/11/1799    most recent
pci194v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Wu, X.
Right arrow Articles by Schreiber, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, X.
Right arrow Articles by Schreiber, L.
Agricola
Right arrow Articles by Wu, X.
Right arrow Articles by Schreiber, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received April 5, 2005
Accepted August 22, 2005

Regular Paper

Casparian Strips in Needles Are More Solute-Permeable than Endodermal Transport Barriers in Roots of Pinus bungeana

Xiaoqin Wu 1, Jinxing Lin 2*, Qingqing Lin 2, Jian Wang 2, and Lukas Schreiber 3

1 Key Laboratory of Photosynthesis and Molecular Environmental Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093 China; Graduate School of the Chinese Academy of Sciences, Beijing, 100043, China
2 Key Laboratory of Photosynthesis and Molecular Environmental Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093 China
3 Department of Ecophysiology, IZMB, University of Bonn, Kirschallee 1, Bonn, 53115, Germany

* To whom correspondence should be addressed.
Jinxing Lin, E-mail: linjx{at}ibcas.ac.cn


  Abstract

Structure and development of endodermal Casparian strips in Pinus bungeana needles and roots were studied by scanning electron microscopy and fluorescence microscopy. Primary pit fields (PF) frequently occurred in radial walls of Casparian strips isolated from needles, whereas PF were never detected in Casparian strips from roots. Formation of Casparian strips in needles as well as roots started at the outer parts of the radial walls and they finally occupied the entire radial walls of the endodermis. Fourier transform infrared (FTIR) spectroscopy of Casparian strips isolated from roots revealed significant absorption bands characteristic for suberin. However, in Casparian strips of needles rarely evidence for suberin was detected by FTIR spectroscopy. Apoplastic permeability of Casparian strips in needles and roots were probed by the apoplastic tracers, calcofluor and berberine. Casparian strips in roots efficiently blocked the apoplastic transport (AT) of calcofluor and berberine. Casparian strips in needles blocked the AT of calcofluor, but diffusion of berberine was not inhibited and berberine-thiocyanate crystals were detectable in the vascular tissue of the needles. From the data presented it must be concluded that Casparian strips in needles, which are characterized by the absence of suberin, are more solute-permeable compared to Casparian strips in roots.

Keywords: Apoplast; Casparian strips; Endodermis; FTIR spectroscopy; Permeability; Pinus bungeana.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.