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Plant and Cell Physiology Advance Access published online on August 24, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci188
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received April 20, 2005
Accepted August 10, 2005

Regular Paper

Localization of NAD-Isocitrate Dehydrogenase and Glutamate Dehydrogenase in Rice Roots: Candidates for Providing Carbon Skeletons to NADH-Glutamate Synthase

Tomomi Abiko 1, Mitsuhiro Obara 2, Akiko Ushioda 1, Toshihiko Hayakawa 1, Michael Hodges 3, and Tomoyuki Yamaya 2*

1 Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
2 Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan; CREST, JST (Japan Science and Technology Agency)
3 Institut de Biotechnologie des Plantes (UMR8618 CNRS), Bâtiment 630, Université de Paris Sud-XI, 91405 Orsay cedex, France

* To whom correspondence should be addressed.
Tomoyuki Yamaya, E-mail: tyamaya{at}biochem.tohoku.ac.jp


  Abstract

In rice roots, transient and cell-type specific accumulation of both mRNA and protein for NADH-dependent glutamate synthase (NADH-GOGAT) occurs after the supply of NH4+ ions (Ishiyama et al. 1998, 2003). In order to better understand the origin of 2-oxoglutarate for this reaction, we focused on mitochondrial NAD-dependent isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GDH) in rice roots. Six rice cDNAs encoding a single catalytic (OsIDHa) and two regulatory (OsIDHc;1, OsIDHc;2) IDH subunits and three GDH proteins (OsGDH1 to 3) were isolated. These genes, except OsGDH3, were expressed in the roots. Real time PCR analysis showed that OsIDHa and OsIDHc;1 transcripts, but not OsGDH1 and OsGDH2 transcripts, accumulated in a similar manner to NADH-GOGAT mRNA along the crown roots after the supply of different forms of inorganic nitrogen. Furthermore, immunolocalization studies revealed the NH4+ induction of IDHa protein in two cell-layers of the root surface, i.e. epidermis and exodermis, where NADH-GOGAT also accumulated (Ishiyama et al. 1998). The possible relationship between NADH-GOGAT, IDH and GDH are discussed.

Keywords: 2-oxoglutarate; Carbon and nitrogen metabolisms; Glutamate dehydrogenase; Isocitrate dehydrogenase; NADH-Glutamate synthase; Rice.
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