Skip Navigation



Plant and Cell Physiology Advance Access published online on July 20, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci172
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Supplementary Data
Right arrow supplementary data
Right arrow All Versions of this Article:
46/9/1568    most recent
pci172v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Sakurai, J.
Right arrow Articles by Maeshima, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sakurai, J.
Right arrow Articles by Maeshima, M.
Agricola
Right arrow Articles by Sakurai, J.
Right arrow Articles by Maeshima, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received June 19, 2005
Accepted July 12, 2005

Regular Paper

Identification of 33 Rice Aquaporin Genes and Analysis of Their Expression and Function

Junko Sakurai 1*, Fumiyoshi Ishikawa 2, Tomoya Yamaguchi 3, Matsuo Uemura 4, and Masayoshi Maeshima 2

1 CO2 & Temperature Research Laboratory, National Agricultural Research Center for Tohoku Region, Morioka, 020-0198 Japan; United Graduate School of Agricultural Sciences and Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, Morioka, 020-8550 Japan
2 Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan
3 CO2 & Temperature Research Laboratory, National Agricultural Research Center for Tohoku Region, Morioka, 020-0198 Japan
4 United Graduate School of Agricultural Sciences and Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, Morioka, 020-8550 Japan

* To whom correspondence should be addressed.
Junko Sakurai, E-mail: junkoi{at}affrc.go.jp


  Abstract

Plant aquaporins form a large protein family including plasma-membrane-type (PIP) and tonoplast-type aquaporins (TIP) and facilitate the osmotic water transport across membranes as a key physiological function. We identified 33 genes for aquaporins in the genome sequence of rice (Oryza sativa L. cv. Nipponbare). We investigated their expression levels in leaf blades, roots and anthers of rice (cv. Akitakomachi) using the semi-quantitative RT-PCR. At both early tillering (21 days after germination) and panicle formation (56 days) stages, six genes, such as OsPIP2;4 and OsPIP2;5, were predominantly expressed in roots, while 14 genes, such as OsPIP2;7 and OsTIP1;2, were in leaf blades. Eight genes, such as OsPIP1;1 and OsTIP4;1, were evenly expressed in leaf blades, roots and anthers. Analysis by stopped flow spectrophotometry revealed high water channel activity when OsPIP2;4 or OsPIP2;5 was expressed in yeast but not when OsPIP1;1 or OsPIP1;2 was expressed. Furthermore, the mRNA levels of OsPIP2;4 and OsPIP2;5 showed a clear diurnal fluctuation in roots; they showed a peak 3 h after the onset of light and dropped to a minimum 3 h after the onset of darkness. The mRNA levels of 10 genes including OsPIP2;4 and OsPIP2;5 markedly decreased in roots during chilling treatment and recovered after warming. The changes in mRNA levels during and after the chilling treatment were comparable to that of the bleeding sap volume. These results suggested the relationship between the root water uptake and mRNA levels of several aquaporins with high water channel activity, such as OsPIP2;4 and OsPIP2;5.

Keywords: Aquaporin; Gene expression; Rice; Water permeability.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
M. C. Voicu, J. E. K. Cooke, and J. J. Zwiazek
Aquaporin gene expression and apoplastic water flow in bur oak (Quercus macrocarpa) leaves in relation to the light response of leaf hydraulic conductance
J. Exp. Bot., October 1, 2009; 60(14): 4063 - 4075.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R.-Y. Li, Y. Ago, W.-J. Liu, N. Mitani, J. Feldmann, S. P. McGrath, J. F. Ma, and F.-J. Zhao
The Rice Aquaporin Lsi1 Mediates Uptake of Methylated Arsenic Species
Plant Physiology, August 1, 2009; 150(4): 2071 - 2080.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. B. Heinen, Q. Ye, and F. Chaumont
Role of aquaporins in leaf physiology
J. Exp. Bot., July 1, 2009; 60(11): 2971 - 2985.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
A. K. Azad, Y. Sawa, T. Ishikawa, and H. Shibata
Heterologous Expression of Tulip Petal Plasma Membrane Aquaporins in Pichia pastoris for Water Channel Analysis
Appl. Envir. Microbiol., May 1, 2009; 75(9): 2792 - 2797.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Matsumoto, H.-L. Lian, W.-A. Su, D. Tanaka, C. w. Liu, I. Iwasaki, and Y. Kitagawa
Role of the Aquaporin PIP1 Subfamily in the Chilling Tolerance of Rice
Plant Cell Physiol., February 1, 2009; 50(2): 216 - 229.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G.-W. Li, M.-H. Zhang, W.-M. Cai, W.-N. Sun, and W.-A. Su
Characterization of OsPIP2;7, a Water Channel Protein in Rice
Plant Cell Physiol., December 1, 2008; 49(12): 1851 - 1858.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Murai-Hatano, T. Kuwagata, J. Sakurai, H. Nonami, A. Ahamed, K. Nagasuga, T. Matsunami, K. Fukushi, M. Maeshima, and M. Okada
Effect of Low Root Temperature on Hydraulic Conductivity of Rice Plants and the Possible Role of Aquaporins
Plant Cell Physiol., September 1, 2008; 49(9): 1294 - 1305.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. K. Azad, M. Katsuhara, Y. Sawa, T. Ishikawa, and H. Shibata
Characterization of Four Plasma Membrane Aquaporins in Tulip Petals: A Putative Homolog is Regulated by Phosphorylation
Plant Cell Physiol., August 1, 2008; 49(8): 1196 - 1208.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. F. Ma, N. Yamaji, N. Mitani, X.-Y. Xu, Y.-H. Su, S. P. McGrath, and F.-J. Zhao
Transporters of arsenite in rice and their role in arsenic accumulation in rice grain
PNAS, July 22, 2008; 105(29): 9931 - 9935.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. J. Norton, D. E. Lou-Hing, A. A. Meharg, and A. H. Price
Rice-arsenate interactions in hydroponics: whole genome transcriptional analysis
J. Exp. Bot., May 2, 2008; (2008) ern097v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Mahdieh, A. Mostajeran, T. Horie, and M. Katsuhara
Drought Stress Alters Water Relations and Expression of PIP-Type Aquaporin Genes in Nicotiana tabacum Plants
Plant Cell Physiol., May 1, 2008; 49(5): 801 - 813.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Suzuki, K. Nagasuga, and M. Okada
The Chilling Injury Induced by High Root Temperature in the Leaves of Rice Seedlings
Plant Cell Physiol., March 1, 2008; 49(3): 433 - 442.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J. Sakurai, A. Ahamed, M. Murai, M. Maeshima, and M. Uemura
Tissue and Cell-Specific Localization of Rice Aquaporins and Their Water Transport Activities
Plant Cell Physiol., January 1, 2008; 49(1): 30 - 39.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J. Y. Jang, J. Y. Rhee, D. G. Kim, G. C. Chung, J. H. Lee, and H. Kang
Ectopic Expression of a Foreign Aquaporin Disrupts the Natural Expression Patterns of Endogenous Aquaporin Genes and Alters Plant Responses to Different Stress Conditions
Plant Cell Physiol., September 1, 2007; 48(9): 1331 - 1339.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W.-G. Choi and D. M. Roberts
Arabidopsis NIP2;1, a Major Intrinsic Protein Transporter of Lactic Acid Induced by Anoxic Stress
J. Biol. Chem., August 17, 2007; 282(33): 24209 - 24218.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
W. Wei, E. Alexandersson, D. Golldack, A. J. Miller, P. O. Kjellbom, and W. Fricke
HvPIP1;6, a Barley (Hordeum vulgare L.) Plasma Membrane Water Channel Particularly Expressed in Growing Compared with Non-Growing Leaf Tissues
Plant Cell Physiol., August 1, 2007; 48(8): 1132 - 1147.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Koizumi, T. Ookawa, H. Satoh, and T. Hirasawa
A Wilty Mutant of Rice has Impaired Hydraulic Conductance
Plant Cell Physiol., August 1, 2007; 48(8): 1219 - 1228.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Li, W. Xu, W. Yang, Z. Kong, and Y. Xue
Genome-Wide Gene Expression Profiling Reveals Conserved and Novel Molecular Functions of the Stigma in Rice
Plant Physiology, August 1, 2007; 144(4): 1797 - 1812.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Yamaji and J. F. Ma
Spatial Distribution and Temporal Variation of the Rice Silicon Transporter Lsi1
Plant Physiology, March 1, 2007; 143(3): 1306 - 1313.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Martinoia, M. Maeshima, and H. E. Neuhaus
Vacuolar transporters and their essential role in plant metabolism
J. Exp. Bot., January 1, 2007; 58(1): 83 - 102.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
R. AROCA, A. FERRANTE, P. VERNIERI, and M. J. CHRISPEELS
Drought, Abscisic Acid and Transpiration Rate Effects on the Regulation of PIP Aquaporin Gene Expression and Abundance in Phaseolus vulgaris Plants
Ann. Bot., December 1, 2006; 98(6): 1301 - 1310.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Mizutani, S. Watanabe, T. Nakagawa, and M. Maeshima
Aquaporin NIP2;1 is Mainly Localized to the ER Membrane and Shows Root-Specific Accumulation in Arabidopsis thaliana
Plant Cell Physiol., October 1, 2006; 47(10): 1420 - 1426.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.