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Plant and Cell Physiology Advance Access published online on July 20, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci169
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received April 14, 2005
Accepted July 11, 2005

Regular Paper

A Suppressor Mutation in {alpha}-Phycocyanin Gene in Light/Glucose-Sensitive Phenotype of psbK-Disruptant of the Cyanobacterium Synechocystis sp. PCC 6803

Mari Kobayashi 1, Katsuhiko Okada 2, and Masahiko Ikeuchi 1*

1 Department of Life Sciences (Biology), The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153-8902, Japan
2 School of Life Science, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo, Japan

* To whom correspondence should be addressed.
Masahiko Ikeuchi, E-mail: mikeuchi{at}bio.c.u-tokyo.ac.jp.


  Abstract

psbK encodes a small transmembrane component of PSII. Here we report that the psbK-disruptant of Synechocystis sp. PCC 6803 cannot survive under photomixotrophic conditions of light and glucose after transient growth, while wild type is able to grow. A spontaneous yellow-green mutant that recovered the sustained growth under the same condition was isolated from the psbK-disruptant. Instead of the recovery, the mutant largely lost the photoautotrophic growth. By phenotype complementation, the mutation was identified in cpcA as sequence replacement with a close downstream segment, generating inverted repeat of 23 bp. The mutant phenotype was featured by (1) the complete loss of {alpha}- and {beta}-phycocyanin, (2) increased accumulation of PSII, (3) greatly reduced transcripts harboring cpcA in abundance and in size. The inverted repeat generated in cpcA probably led to the early termination of transcription. Possible mechanism for such mutation is discussed.

Keywords: cpcA; light/glucose-sensitive; phycocyanin; psbK; sequence substitution; template switching.
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