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Plant and Cell Physiology Advance Access published online on July 16, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci165
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received May 6, 2005
Accepted July 7, 2005

Regular Paper

The Effect of Over-Expression of Two Brassica CBF/DREB1-Like Transcription Factors on Photosynthetic Capacity and Freezing Tolerance in Brassica napus

Leonid V. Savitch 1, Ghislaine Allard 1, Motoaki Seki 2, Laurian S. Robert 1, Nicholas A. Tinker 1, Norman P. A. Huner 3, Kazuo Shinozaki 2, and Jas Singh 1*

1 Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Central Experimental Farm, Ottawa, Ontario, Canada, K1A 0C6
2 Biological Resources Division, Japan International Research Centre for Agricultural Sciences, 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan
3 Department of Biology and the Biotron, University of Western Ontario, London, ON, Canada, N6A 5B7

* To whom correspondence should be addressed.
Jas Singh, E-mail: singhja{at}agr.gc.ca


  Abstract

The effects of overexpression of two Brassica CBF/DREB1-like transcription factors (BNCBF5, 17; Gao et al. 2002) in Brassica napus cv. Westar were studied. In addition to developing constitutive freezing tolerance and constitutively accumulate COR gene mRNAs, BNCBF5 and 17oe plants also accumulate moderate transcript levels of genes involved in photosynthesis and chloroplast development as identified by microarray and northern analyses. These include GLK1 and GLK2-like transcription factors involved in chloroplast photosynthetic development, chloroplast stroma cyclophilin ROC4 (AtCYP20-3), {beta}-amylase and Triose-P/Pi translocator. These changes are paralleled by observations of increases in photosynthetic efficiency and capacity, pigment pool sizes, increased capacities of the Calvin cycle enzymes, enzymes of starch and sucrose biosynthesis as well as glycolysis and oxaloacetate/malate exchange, suggesting that BNCBF overexpression have partially mimicked cold-induced photosynthetic acclimation constitutively. Taken together, these results suggest that BNCBF/DREB1oe in Brassica not only resulted in increased constitutive freezing tolerance but also partially regulated chloroplast development to increase photochemical efficiency and photosynthetic capacity.

Keywords: Brassica; CBF/DREB1; cold acclimation; freezing tolerance; photosynthesis; GLK.
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