Skip Navigation



Plant and Cell Physiology Advance Access published online on June 17, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci155
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
46/9/1452    most recent
pci155v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Suzuki, T.
Right arrow Articles by Nakamura, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suzuki, T.
Right arrow Articles by Nakamura, K.
Agricola
Right arrow Articles by Suzuki, T.
Right arrow Articles by Nakamura, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received December 22, 2004
Accepted June 13, 2005

Regular Paper

An Arabidopsis Protein with a Novel Calcium-Binding Repeat Sequence Interacts with TONSOKU/MGOUN3/BRUSHY1 Involved in Meristem Maintenance

Takamasa Suzuki 1*, Sakiko Nakajima 1, Atsushi Morikami 2, and Kenzo Nakamura 1

1 Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan
2 Faculty of Agriculture, Meijo University, Tenpaku-ku, Nagoya 468-8502, Japan

* To whom correspondence should be addressed.
Takamasa Suzuki, E-mail: takamasa{at}thaliana.myhome.cx


  Abstract

TONSOKU(TSK)/MGOUN3/BRUSHY1 from Arabidopsis thaliana, which plays an important role in the maintenance of meristem organization, contains an LGN repeat motif similar to that found in animal proteins involved in asymmetric cell division. One protein that interacts with the LGN motif of TSK in a yeast two-hybrid screen, TSK-Associating Protein 1 (TSA1), contains a 10-fold repeat of a unique 41-amino-acid sequence. The repeat sequence, with a glutamic acid-phenylalanine-glutamic acid (EFE) conserved core sequence, is enriched with acidic amino acids. TSA1 also contains an N-terminal putative signal peptide and it interacts with the LGN motif of TSK through a C-terminal region separated from the EFE repeats by a putative membrane-spanning region. The recombinant protein consisting of EFE repeats was rich in {alpha}-helical structure and possessed Ca2+-binding activity. Unlike nuclear localization of TSK, the TSA1 fused with green fluorescent protein (GFP) expressed in tobacco BY-2 cells was localized in small cytoplasmic vesicles during interphase. However, cellular localization of both TSA1-GFP and GFP-TSK changed dynamically during mitosis. In particular, both GFP-TSK and TSA1-GFP were concentrated in limited areas that are close to ends of spindle microtubules ahead of separating chromatids. These results are discussed in terms of the possible involvement of TSK and TSA1 in mitosis.

Keywords: tonsoku/brushy1/mgoun3; calcium-binding protein; EFE repeat; mitosis; Arabidopsis thaliana.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
K. Yamada, A. J. Nagano, M. Nishina, I. Hara-Nishimura, and M. Nishimura
NAI2 Is an Endoplasmic Reticulum Body Component That Enables ER Body Formation in Arabidopsis thaliana
PLANT CELL, September 1, 2008; 20(9): 2529 - 2540.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. J. Nagano, Y. Fukao, M. Fujiwara, M. Nishimura, and I. Hara-Nishimura
Antagonistic Jacalin-Related Lectins Regulate the Size of ER Body-Type {beta}-Glucosidase Complexes in Arabidopsis thaliana
Plant Cell Physiol., June 1, 2008; 49(6): 969 - 980.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Inagaki, T. Suzuki, M.-a. Ohto, H. Urawa, T. Horiuchi, K. Nakamura, and A. Morikami
Arabidopsis TEBICHI, with Helicase and DNA Polymerase Domains, Is Required for Regulated Cell Division and Differentiation in Meristems
PLANT CELL, April 1, 2006; 18(4): 879 - 892.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.