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Plant and Cell Physiology Advance Access published online on May 26, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci134
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received June 7, 2004
Accepted May 19, 2005

Regular Paper

Inducible Antisense-Mediated Post-Transcriptional Gene Silencing in Transgenic Pine Cells Using Green Fluorescence Protein as a Visual Marker

Wei Tang 1*, Katherine Kinken 1, and Ronald J. Newton 1

1 Department of Biology, Howell Science Complex, East Carolina University, Greenville, NC 27858-4353, USA

* To whom correspondence should be addressed.
Wei Tang, E-mail: tangw{at}mail.ecu.edu


  Abstract

An inducible post-transcriptional gene silencing (PTGS) system was established in Virginia pine (Pinus virginiana Mill.) cells. This system is based on the activation of an anti-sense gfp gene construct by a chimaeric transcriptional activator GVG upon application of the inducer to gfp transgenic cell lines. A detailed characterization of the inducible post-transcriptional gene silencing (PTGS) system in transgenic cell lines demonstrated that this system is stringently controlled. The degree of silencing with this construct could be regulated by the concentration of inducer and the time of treatment. Such transgenic cell lines may provide a useful system to study signaling mechanisms of gene silencing in transgenic pine cells. The inducible system could be a useful tool for functional discovery of novel plant genes.

Keywords: Chimaeric transcription factor; conditional gene expression; dexamethasone; post-transcriptional gene silencing; RNA interference; transgene.
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