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Plant and Cell Physiology Advance Access published online on May 26, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci132
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received March 18, 2005
Accepted May 17, 2005

Regular Paper

Identification of Low-Temperature Regulated ORFs in the Cyanobacterium Anabaena sp. Strain PCC 7120: Distinguishing the Effects of Low Temperature from the Effects of Photosystem II Excitation Pressure

Shigeki Ehira 1, Masayuki Ohmori 1, and Naoki Sato 1*

1 Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University, 255 Shimo-Ohkubo, Sakura, Saitama 338-8570, Japan; Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902, Japan

* To whom correspondence should be addressed.
Naoki Sato, E-mail: naokisat{at}bio.c.u-tokyo.ac.jp


  Abstract

Most organisms have developed various strategy to react rapidly to temperature downshift and regulate expression of various genes to acclimate to low temperature. In photosynthetic organisms, temperature downshift in the light results in not only decrease in growth temperature but also increase in photosystem II excitation pressure. Distinguishing the effects of low temperature from the effects of excitation pressure is necessary for understanding the mechanism of low-temperature signal transduction. In this report, we analyzed changes in gene expression after three different environmental changes, i.e. temperature downshift in the light, temperature downshift in the dark and transfer to the dark, using DNA microarray in the cyanobacterium Anabaena sp. strain PCC 7120. By comparing the expression patterns under the three experimental conditions, we identified 15 ORFs that were upregulated by temperature downshift both in the light and in the dark. These ORFs are considered to be regulated by low temperature, but not by excitation pressure. Six of them have a consensus sequence within the upstream region of their coding region and were indicated to be also upregulated by tetracycline. Functional or structural changes in ribosome could affect transcript levels of the low-temperature regulated ORFs.

Keywords: Anabaena sp. PCC 7120; cyanobacteria; DNA microarray; excitation pressure; low temperature.
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