Skip Navigation



Plant and Cell Physiology Advance Access published online on May 20, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci130
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
46/8/1213    most recent
pci130v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Prassinos, C.
Right arrow Articles by Han, K.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Prassinos, C.
Right arrow Articles by Han, K.-H.
Agricola
Right arrow Articles by Prassinos, C.
Right arrow Articles by Han, K.-H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received September 22, 2004
Accepted May 16, 2005

Regular Paper

Transcriptome Profiling of Vertical Stem Segments Provides Insights into the Genetic Regulation of Secondary Growth in Hybrid Aspen Trees

Constantinos Prassinos 1, Jae-Heung Ko 1, Jaemo Yang 1, and Kyung-Hwan Han 1*

1 Department of Forestry, Michigan State University, 126 Natural Resources, East Lansing, MI 48824-1222

* To whom correspondence should be addressed.
Kyung-Hwan Han, E-mail: hanky{at}msu.edu


  Abstract

In order to better understand the genetic regulation of secondary growth in hybrid aspen (Populus tremula L. x P. alba L.), we carried out a series of cDNA-AFLP-based transcriptome analyses in vertical stem segments that represent a gradient of developmental stages with regard to secondary growth. This approach allowed us to screen over 80% of the transcriptome expressed in six samples and identify genes differentially expressed with the progress of secondary growth, in a tissue specific manner. Of the 76,800 transcript-derived fragments (TDFs) analyzed, 271 TDFs were selected and sequenced based on their differential expression patterns. Many of the xylem-upregulated genes were involved in cell wall and lignin biosynthesis, while the bark-upregulated genes had diverse functional roles. About 25% of the xylem-upregulated TDFs analyzed were involved in the phenylpropanoid biosynthesis pathway, which produces the cell wall polymer lignin and various wood extractives. In addition, many of the TDFs showing secondary xylem-specific expression were annotated as genes not previously reported in Populus, including novel cell death proteins, cytoskeleton interacting proteins, transporters and putative transcription factors.

Keywords: cDNA-AFLP; Populus; secondary growth; stem; wood formation.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Tree PhysiolHome page
K. Sato, N. Nishikubo, Y. Mashino, K. Yoshitomi, J. Zhou, S. Kajita, and Y. Katayama
Immunohistochemical localization of enzymes that catalyze the long sequential pathways of lignin biosynthesis during differentiation of secondary xylem tissues of hybrid aspen (Populus sieboldii x Populus grandidentata)
Tree Physiol, December 1, 2009; 29(12): 1599 - 1606.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
M. J. Roach and M. K. Deyholos
Microarray Analysis of Developing Flax Hypocotyls Identifies Novel Transcripts Correlated with Specific Stages of Phloem Fibre Differentiation
Ann. Bot., September 1, 2008; 102(3): 317 - 330.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Baucher, M. El Jaziri, and O. Vandeputte
From primary to secondary growth: origin and development of the vascular system
J. Exp. Bot., October 1, 2007; 58(13): 3485 - 3501.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.