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Plant and Cell Physiology Advance Access published online on May 20, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci130
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received September 22, 2004
Accepted May 16, 2005

Regular Paper

Transcriptome Profiling of Vertical Stem Segments Provides Insights into the Genetic Regulation of Secondary Growth in Hybrid Aspen Trees

Constantinos Prassinos 1, Jae-Heung Ko 1, Jaemo Yang 1, and Kyung-Hwan Han 1*

1 Department of Forestry, Michigan State University, 126 Natural Resources, East Lansing, MI 48824-1222

* To whom correspondence should be addressed.
Kyung-Hwan Han, E-mail: hanky{at}msu.edu


  Abstract

In order to better understand the genetic regulation of secondary growth in hybrid aspen (Populus tremula L. x P. alba L.), we carried out a series of cDNA-AFLP-based transcriptome analyses in vertical stem segments that represent a gradient of developmental stages with regard to secondary growth. This approach allowed us to screen over 80% of the transcriptome expressed in six samples and identify genes differentially expressed with the progress of secondary growth, in a tissue specific manner. Of the 76,800 transcript-derived fragments (TDFs) analyzed, 271 TDFs were selected and sequenced based on their differential expression patterns. Many of the xylem-upregulated genes were involved in cell wall and lignin biosynthesis, while the bark-upregulated genes had diverse functional roles. About 25% of the xylem-upregulated TDFs analyzed were involved in the phenylpropanoid biosynthesis pathway, which produces the cell wall polymer lignin and various wood extractives. In addition, many of the TDFs showing secondary xylem-specific expression were annotated as genes not previously reported in Populus, including novel cell death proteins, cytoskeleton interacting proteins, transporters and putative transcription factors.

Keywords: cDNA-AFLP; Populus; secondary growth; stem; wood formation.
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