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Plant and Cell Physiology Advance Access published online on May 12, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci125
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received August 20, 2004
Accepted May 6, 2005

Regular Paper

PeMADS6, a GLOBOSA/PISTILLATA-like Gene in Phalaenopsis equestris Involved in Petaloid Formation, and Correlated with Flower Longevity and Ovary Development

Wen-Chieh Tsai 1, Pei-Fang Lee 2, Hong-Ie Chen 2, Yu-Yun Hsiao 1, Wan-Ju Wei 2, Zhao-Jun Pan 1, Ming-Hsiang Chuang 1, Chang-Sheng Kuoh 1, Wen-Huei Chen 3, and Hong-Hwa Chen 4*

1 Department of Life Sciences, National Cheng Kung University, Tainan 701, Taiwan
2 Department of Biotechnology, Fooyin University, Kaohsiung County 831, Taiwan
3 Department of Life Sciences, National University of Kaohsiung, Kaohsiung 811, Taiwan
4 Department of Life Sciences, National Cheng Kung University, Tainan 701, Taiwan; Institute of Biotechnology, National Cheng Kung University, Tainan 701, Taiwan; Institute of Botany, Academia Sinica, Taipei 115, Taiwan

* To whom correspondence should be addressed.
Hong-Hwa Chen, E-mail: hhchen{at}mail.ncku.edu.tw


  Abstract

In this study, we isolated and characterized the function of a GLOBOSA/PISTILLATA-like gene, PeMADS6, from a native Phalaenopsis species, P. equestris. Southern blot analysis showed PeMADS6 as a single copy in the Phalaenopsis genome. Results of temporal and spatial expressions showed that PeMADS6 was expressed and thus participated in the development of the sepals, petals, labellum and column in Phalaenopsis. Further confirmation of the expression pattern of PeMADS6 was carried out with in situ hybridization. Repressed expression of PeMADS6 in the orchid ovary was found to be pollination regulated, which suggests that the gene may have an inhibitory effect on the development of the ovary or ovule. In addition, auxin acted as the candidate signal to regulate the repression of PeMADS6 expression in the ovary. Furthermore, the flowers of transgenic Arabidopsis plants ectopically overexpressing PeMADS6 showed the morphology of petaloid sepals, with a 3- to 4-fold increase in flower longevity. Concomitantly, delayed fruit maturation was also observed in the transgenic Arabidopsis, which is consistent with the inhibitory effect of PeMADS6 on the development of the ovary. Thus, as a B-function gene, PeMADS6 not only specifies floral organ identity but has functions in flower longevity and ovary development in orchids.

Keywords: flower longevity; floral organ identity; GLOBOSA/PISTILLATA-like gene; ovary/ovule development; overexpression; Phalaenopsis.
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