Skip Navigation



Plant and Cell Physiology Advance Access published online on May 13, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci124
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
46/7/1116    most recent
pci124v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Solfanelli, C.
Right arrow Articles by Picciarelli, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Solfanelli, C.
Right arrow Articles by Picciarelli, P.
Agricola
Right arrow Articles by Solfanelli, C.
Right arrow Articles by Picciarelli, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received January 24, 2005
Accepted May 5, 2005

Regular Paper

Expression of Two Genes Encoding Gibberellin 2- and 3-Oxidases in Developing Seeds of Phaseolus coccineus

Cinzia Solfanelli 1, Francesca Ceron 1, Fabio Paolicchi 1, Lucia Giorgetti 2, Chiara Geri 2, Nello Ceccarelli 1, Yuji Kamiya 3, and Piero Picciarelli 1*

1 Dipartimento di Biologia delle Piante Agrarie, Università di Pisa, Via Mariscoglio, 34, 56124 Pisa, Italy
2 Istituto di Biologia e Biotecnologie Agrarie, Area Ricerca CNR, via Moruzzi, 1, 56124 Pisa, Italy
3 Plant Science Center, RIKEN, Suehiro-cho 1-7-22, Tsurumi-ku, Yokohama 230-0045, Japan

* To whom correspondence should be addressed.
Piero Picciarelli, E-mail: picciarelli{at}agr.unipi.it


  Abstract

We have isolated PcGA3ox1, a cDNA clone from developing runner bean (Phaseolus coccineus) seeds that shows significant amino-acid homology with the gibberellin (GA) 3-oxidases. A recombinant fusion protein of PcGA3ox1 converted GA20 and GA9 to GA1 and GA4, respectively. In situ hybridization results showed that transcripts of this gene accumulate specifically within the suspensor of globular-stage embryos. PcGA3ox1 mRNA begins to accumulate in the epidermal cells of the embryo proper and is also detectable in the endosperm during the transition from globular- to heart-stage embryos. PcGA3ox1 transcripts were localized exclusively in the cotyledons from the early cotyledonary stage up to the cotyledonary stage. Transcripts of the previously cloned GA 2-oxidase (PcGA2ox1) (Thomas et al. 1999) from developing seeds of runner bean were found primarily within the suspensor neck region from the late globular stage up to the heart stage. PcGA2ox1 mRNA was detectable in the whole suspensor from the early cotyledonary stage, and was found in the inner layer of integuments at the cotyledonary stage. Soluble enzyme preparations made from suspensors and embryos at two stages of embryogenesis (the heart and cotyledonary stages) were incubated with [14C]GA20 and [14C]GA1. Only young suspensor preparations converted GA20 to GA1 and GA5. Both suspensor preparations converted GA1 to GA8. Both embryo preparations converted GA20 to GA1, but were unable to convert GA1 to GA8.

Keywords: developing seed; GA 2-oxidase; GA 3-oxidase; gibberellin; runner bean.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.