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Plant and Cell Physiology Advance Access published online on May 3, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci121
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received November 10, 2004
Accepted April 28, 2005

Regular Paper

Caffeine Inhibits the Callose Deposition in the Cell Plate and the Depolymerization of Microtubules in the Central Region of the Phragmoplast

Hiroki Yasuhara 1*

1 Department of Biotechnology, Faculty of Engineering and High Technology Research Center (HRC), Kansai University, Yamate-cho, Suita, Osaka 564-8680, Japan

* To whom correspondence should be addressed.
Hiroki Yasuhara, E-mail: yasuhara{at}ipcku.kansai-u.ac.jp


  Abstract

Treatment of tobacco BY-2 cells with 10 mM caffeine that was started after the cells had entered the mitotic phase did not completely inhibit the deposition of callose in the cell plate and allowed the centrifugal redistribution of phragmoplast microtubules. On the other hand, when treatment with caffeine was started before the cells entered the mitotic phase, the deposition of callose was completely inhibited and the redistribution of phragmoplast microtubules was also inhibited. As the inhibition of redistribution of phragmoplast microtubules seems to be caused by the inhibition of depolymerization of microtubules at the central region of the phragmoplast (Yasuhara et al. 1993), these results strongly suggest that the deposition of callose in the cell plate is tightly linked with the depolymerization of phragmoplast microtubules. Callose deposition was observed in phragmoplasts isolated from caffeine treated cells as was in those isolated from caffeine-untreated cells and caffeine did not inhibit callose synthesis in isolated phragmoplast, indicating that caffeine neither inhibits the accumulation of callose synthase at the equatorial regions of the phragmoplast nor arrest callose synthase itself.

Keywords: Caffeine; Cell plate; Callose; Cytokinesis; Phragmoplast; Tobacco BY-2 cells.
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