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Plant and Cell Physiology Advance Access published online on April 19, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci109
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received September 2, 2004
Accepted April 9, 2005

Regular Paper

A Peroxiredoxin Q Homolog from Gentians Is Involved in Both Resistance against Fungal Disease and Oxidative Stress

Akinori Kiba 1*, Masahiro Nishihara 2, Nobue Tsukatani 2, Takashi Nakatsuka 2, Yoshiaki Kato 3, and Saburo Yamamura 2

1 Iwate Biotechnology Research Center, Kitakami, Iwate 024-0003, Japan; Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University, Nankoku 783-8502, Japan
2 Iwate Biotechnology Research Center, Kitakami, Iwate 024-0003, Japan
3 Iwate Biotechnology Research Center, Kitakami, Iwate 024-0003, Japan; Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, 3-18-8, Morioka, Iwate 020-8550, Japan

* To whom correspondence should be addressed.
Akinori Kiba, E-mail: akiba{at}cc.kochi-u.ac.jp


  Abstract

An antifungal protein (GtAFP1) showing antimicrobial activity against phytopathogenic fungi was purified from leaves of Gentiana triflora. The deduced amino acid sequence of the cDNA of the corresponding gene, GtAFP1, showed 94, 75, 72 and 63 % amino acid identities with peroxiredoxin Q from Populus balsamifera x P. deltoides subsp. trichocarpa, Sedum lineare, Suaeda maritima and Arabidopsis thaliana, respectively. The GtAFP1 gene suggested to be present in the genome in one to two copies and was expressed in the leaves, roots, and stems. Expression of GtAFP1 was induced by treatment with salicylic acid, but not methyl jasmonate. Recombinant GtAFP1 protein not only showed antifungal activity but also thioredoxin-dependent peroxidase activity. Overexpression of GtAFP1 in tobacco plants improved tolerance not only against fungal diseases but also oxidative stress. These results indicate that GtAFP1 might act as a disease and oxidative stress defensive gene in plants and could be useful for engineering stress-resistant plants.

Keywords: Peroxiredoxin Q; antifungal protein; Gentiana triflora; recombinant protein; thioredoxin peroxidase; transgenic tobacco.
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