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Plant and Cell Physiology Advance Access first published online on March 28, 2005
This version published online on March 30, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci096
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received March 22, 2005
Accepted March 22, 2005

Rapid Paper

Regulation of CAPRICE Transcription by MYB Proteins for Root Epidermis Differentiation in Arabidopsis

Yoshihiro Koshino-Kimura 1, Takuji Wada 2, Tatsuhiko Tachibana 3, Ryuji Tsugeki 3, Sumie Ishiguro 3, and Kiyotaka Okada 1*

1 Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
2 Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan
3 Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan

* To whom correspondence should be addressed.
Kiyotaka Okada, E-mail: kiyo{at}ok-lab.bot.kyoto-u.ac.jp


  Abstract

Epidermal-cell differentiation in Arabidopsis root is studied as a model system for understanding cell-fate specification. Two types of MYB-related transcription factors are involved in these cell differentiation. One of these, CAPRICE (CPC), encoding an R3-type MYB protein, is a positive regulator of hair-cell differentiation and is preferentially transcribed in hairless cells. To determine the mechanism of the CPC transcription, we analyzed the regulatory mechanism of the CPC transcription. Deletion analyses of the CPC promoter revealed that hairless-cell-specific transcription of the CPC gene required a sixty-nine bp sequence, and tandem repeat of this region was sufficient for its expression in epidermis. This region includes two MYB-binding sites and the epidermis-specific transcription of CPC was abolished when base substitutions were introduced in these sites. We showed that WEREWOLF (WER), which is an R2R3-type MYB protein, directly bind to this region by gel mobility shift experiments and by yeast one-hybrid assay. We showed that WER also binds to GL2 promoter region, indicating that WER directly regulates the CPC and GL2 transcriptions by binding to their promoter regions.

Keywords: CAPRICE; cell differentiation; DNA binding; MYB protein; transcription factor; WEREWOLF.
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