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Plant and Cell Physiology Advance Access published online on March 25, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci085
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received April 23, 2004
Accepted March 8, 2005

Regular Papar

Peroxisomal Localization of a Myosin XI Isoform in Arabidopsis thaliana

Kohsuke Hashimoto 1*, Hisako Igarashi 1, Shoji Mano 2, Mikio Nishimura 2, Teruo Shimmen 1, and Etsuo Yokota 1

1 Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo, 678-1297, Japan
2 Department of Cell Biology, National Institute for Basic biology, Okazaki, 444-8585, Japan

* To whom correspondence should be addressed.
Kohsuke Hashimoto, E-mail: 0510hk{at}sci.himeji-tech.ac.jp


  Abstract

The genome of Arabidopsis thaliana contains 13 myosin XI isoforms. Here we prepared a specific antibody against a peptide that mimics a unique C-terminal region from the myosin XI isoform, MYA2. The resulting antibody was used to demonstrate that MYA2 in Arabidopsis protein extracts co-sedimented with actin filaments and dissociated from the filaments with an ATP treatment. Immunolocalization studies showed that MYA2 co-localized predominantly with actin filaments in clustered punctuate dots in leaf epidermal cells, root hair cells, and suspension cultured cells. In a transgenic plant in which peroxisomes are labeled with green fluorescent protein, some MYA2 signals were localized on peroxisomes in an actin-dependent manner. We propose that the peroxisome is one of the cargos translocated by MYA2 on actin filaments.

Keywords: Actin; Arabidopsis; MYA 2; Myosin XI; Peroxisome.
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