Skip Navigation



Plant and Cell Physiology Advance Access published online on March 7, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci078
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
46/5/754    most recent
pci078v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Nomura, M.
Right arrow Articles by Tajima, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nomura, M.
Right arrow Articles by Tajima, S.
Agricola
Right arrow Articles by Nomura, M.
Right arrow Articles by Tajima, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received August 8, 2004
Accepted February 20, 2005

Regular Paper

Differential Expression Pattern of C4-Bundle Sheath Expression Genes in Rice, a C3 Plant

Mika Nomura 1*, Tomonori Higuchi 1, Yuji Ishida 2, Shozo Ohta 2, Toshihiko Komari 2, Nobuyuki Imaizumi 3, Mitsue Miyao-Tokutomi 4, Makoto Matsuoka 5, and Shigeyuki Tajima 1

1 Faculty of Agriculture, Kagawa University, Miki, Kita, Kagawa, 761-0795, Japan
2 Japan Tobacco Inc., 700 Higashibara, Toyoda, Iwata, Shizuoka, 438, Japan
3 Japan Turfgrass Inc., 1-1-20, higashi-nihonbashi, Chuo, Tokyo, 103-0004, Japan
4 National Institute of Agrobiological Resources, Tsukuba, 305-8602, Japan
5 BioScience Center, Nagoya University, Chikusa, Nagoya, 464-8601, Japan

* To whom correspondence should be addressed.
Mika Nomura, E-mail: nomura{at}ag.kagawa-u.ac.jp


  Abstract

NADP-malic enzyme (NADP-ME) and phosphoenolpyruvate carboxykinase (PCK) are specifically expressed in bundle sheath cells (BSCs) in NADP-ME-type and PCK-type C4 plants, respectively. Unlike the high activities of these enzymes in the green leaves of C4 plants, their low activities have been detected in the leaves of C3 plants. In order to elucidate the differences in the gene expression system between C3 and C4 plants, we have produced chimeric constructs with the {beta}-glucuronidase (GUS) reporter gene under the control of the maize NADP-Me (ZmMe) or Zoysia japonica Pck (ZjPck) promoter and introduced these constructs into rice. In leaves of transgenic rice, the ZmMe promoter directed GUS expression not only in mesophyll cells (MCs) but also in BSCs and vascular cells, whereas the ZjPck promoter directed GUS expression only in BSCs and vascular cells. Neither the ZjPck nor ZmMe promoters induced GUS expression due to light. In rice leaves, the endogenous NADP-Me (OsMe1) was expressed in MCs, BSCs and vascular cells, whereas the rice Pck (OsPck1) was expressed only in BSCs and vascular cells. Taken together, the results obtained from transgenic rice demonstrate that the expression pattern of ZmMe or ZjPck in transgenic rice was reflected by that of its counterpart gene in rice.

Keywords: C4 plant; NADP-malic enzyme; Phosphoenolpyruvate carboxykinase; Promoter analysis; Rice.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
P.-A. Christin, B. Petitpierre, N. Salamin, L. Buchi, and G. Besnard
Evolution of C4 Phosphoenolpyruvate Carboxykinase in Grasses, from Genotype to Phenotype
Mol. Biol. Evol., February 1, 2009; 26(2): 357 - 365.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Engelmann, C. Wiludda, J. Burscheidt, U. Gowik, U. Schlue, M. Koczor, M. Streubel, R. Cossu, H. Bauwe, and P. Westhoff
The Gene for the P-Subunit of Glycine Decarboxylase from the C4 Species Flaveria trinervia: Analysis of Transcriptional Control in Transgenic Flaveria bidentis (C4) and Arabidopsis (C3)
Plant Physiology, April 1, 2008; 146(4): 1773 - 1785.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Akyildiz, U. Gowik, S. Engelmann, M. Koczor, M. Streubel, and P. Westhoff
Evolution and Function of a cis-Regulatory Module for Mesophyll-Specific Gene Expression in the C4 Dicot Flaveria trinervia
PLANT CELL, November 1, 2007; 19(11): 3391 - 3402.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.