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Plant and Cell Physiology Advance Access published online on March 7, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci077
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received August 8, 2004
Accepted February 20, 2005

Regular Paper

The Promoter for C4-Type Mitochondrial Aspartate Aminotransferase Does Not Direct Bundle Sheath-Specific Expression in Transgenic Rice Plants

Mika Nomura 1*, Tomonori Higuchi 1, Kenichi Katayama 1, Mitsutaka Taniguchi 2, Mitsue Miyao-Tokutomi 3, Makoto Matsuoka 4, and Shigeyuki Tajima 1

1 Faculty of Agriculture, Kagawa University, Miki, Kita, Kagawa, 761-0795 Japan
2 Department of Biological Mechanisms and Function, Nagoya University Chikusa, Nagoya, 464-8601 Japan
3 National Institute of Agrobiological Resources, Tsukuba, 305-8602 Japan
4 BioScience Center, Nagoya University, Chikusa, Nagoya, 464-8601 Japan

* To whom correspondence should be addressed.
Mika Nomura, E-mail: nomura{at}ag.kagawa-u.ac.jp


  Abstract

For NAD-malic enzyme (NAD-Me)-type C4 photosynthesis, two types of aspartate aminotransferase (AAT) are involved. We examined the expression pattern of the Panicum miliaceum mitochondrial Aat gene (PmAat) and P. miliaceum cytosolic Aat gene (PcAat) in transgenic rice plants, which specifically expressed in bundle sheath cells (BSCs) and mesophyll cells (MCs), respectively. Expression of a GUS reporter gene under the control by the PcAat promoter was regulated in an organ-preferential and light-dependent manner in the transgenic rice plants. However, the PmAat promoter drove the GUS expression in all organs we tested without light-dependency and this non-preferential expression pattern was also observed in transgenic rice introducing intact PmAat gene. The expression patterns of the rice counterpart Aat genes to PmAat or PcAat found that rice mitochondrial Aat (RmAat1) gene was expressed in all organs tested in a light-independent manner, while expression of the rice cytosolic Aat (RcAat1) gene showed an organ-preferential and light-dependent manner. Taken together these results, we can generalize that the regulatory system of BSC-specific or light-dependent expression of mitochondrial Aat is not shared between P. miliaceum (C4) and rice (C3) and that the expression of the C4 genes introduced into rice mimic that of their counterpart genes in rice.

Keywords: Aspartate aminotransferase; C4 plant; Promoter analysis; Rice.
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