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Plant and Cell Physiology Advance Access published online on March 8, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci075
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received December 7, 2004
Revised February 7, 2005
Accepted February 14, 2005

Short Communciation

Transcriptional Activity of Male Gamete-Specific Histone gcH3 Promoter in Sperm Cell of Lilium longiflorum

Takashi Okada 1, Prem L. Bhalla 1, and Mohan B. Singh 1*

1 Plant Molecular Biology and Biotechnology laboratory, ARC Centre of Excellence for Integrative Legume Research, Institute of Land and Food Resources, The University of Melbourne, Parkville, Victoria 3010, Australia

* To whom correspondence should be addressed.
Mohan B. Singh, E-mail: mohan{at}unimelb.edu.au


  Abstract

Histones are essential for packaging of eukaryotic genome DNA in nucleosomes and histone gene expression is normally coupled with DNA synthesis. Some of the flowering plant histone genes show strictly male gamete specific expression. However, mechanisms underlying their male gamete-specific expression have not been elucidated so far. Here we report the isolation of male gamete-specific histone gcH3 promoter from Lilium longiflorum and its activity in male gametic cell of flowering plant. OCT motif, which is well conserved in plant histone promoters regulating S phase-specific expression, is not conserved in gcH3 promoter. Instead sequence motifs identical to GC Box 1 and GC box 2, transcriptional activator and suppressor for mammalian testis-specific histone H1t, are present in gcH3 promoter suggesting that plants and animals share the mechanism which govern specificity of gene expression in male gametic cells. Male gamete specific activation of the gcH3 promoter has been confirmed by microprojectile bombardment in lily pollen. The sperm cell carrying gold particles showed reporter gene expression while GFP fluorescence was absent in the other sperm cell which had no particles confirming that gcH3 promoter is activated in male gametic cell and sperm cells have transcriptional and translational machinery that is independent of vegetative cell of pollen.

Keywords: male gamete; generative cell; sperm cell; histone H3; promoter; GFP.
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F. Haerizadeh, M. B. Singh, and P. L. Bhalla
Transcriptional repression distinguishes somatic from germ cell lineages in a plant.
Science, July 28, 2006; 313(5786): 496 - 499.
[Abstract] [Full Text] [PDF]


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