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Plant and Cell Physiology Advance Access published online on March 7, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci073
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received September 14, 2004
Accepted February 11, 2005

Short Communication

Analysis of the Function of the Photoreceptors Phytochrome-B and Phytochrome-D in Nicotiana plumbaginifolia and Arabidopsis thaliana

Aurora Piñas Fernández 1, Patricia Gil 1, Ildiko Valkai 2, Ferenc Nagy 2, and Eberhard Schäfer 1*

1 Albert-Ludwigs-Universität Freiburg, Institut für Biologie II/ Botanik, Schänzlestraße 1, 79104 Freiburg, Germany
2 Institute of Plant Biology, Biological Research Centre, Temesvári krt 62. H-6726 Szeged, Hungary

* To whom correspondence should be addressed.
Eberhard Schäfer, E-mail: eberhard.schaefer{at}biologie.uni-freiburg.de


  Abstract

To investigate the mechanism of phytochrome action in vivo, NtPHYB: and AtPHYB: and phyD:GFP were over-expressed in N. plumbaginifolia and A. thaliana. The expression of 35S:NtPHYB:GFP and 35S:AtPHYB:GFP complemented the tobacco hgl2 and Arabidopsis phyB-9 mutations, whereas the 35S:AtPHYD:GFP only rescued the hgl2 mutant. All three fusion proteins are transported into the nucleus in all genetic backgrounds. These data indicate that (i) AtPHYD:GFP is biologically active and functions as the main red light receptor in transgenic tobacco and (ii) establish an experimental system for the functional analysis of this elusive photoreceptor in vivo.

Keywords: phytochrome; light signalling; Nicotiana plumbaginifolia.
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