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Plant and Cell Physiology Advance Access published online on March 7, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci072
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received January 26, 2005
Accepted February 14, 2005

Rapid Paper

Activator of Spomin::LUC1/WRINKLED1 of Arabidopsis thaliana Transactivates Sugar-Inducible Promoters

Takeshi Masaki 1, Naoko Mitsui 1, Hironaka Tsukagoshi 1, Terumi Nishii 1, Atsushi Morikami 2, and Kenzo Nakamura 1*

1 Laboratory of Biochemistry, Graduate School of Bioagricultural Science, Nagoya University, Chikusa, Nagoya 464-8601, Japan
2 Faculty of Agriculture, Meijo University, Tenpaku, Nagoya 468-8502, Japan

* To whom correspondence should be addressed.
Kenzo Nakamura, E-mail: kenzo{at}agr.nagoya-u.ac.jp


  Abstract

We isolated an enhancer activation-tagged mutant of Arabidopsis thaliana line sGsL carrying luciferase (LUC) gene under control of a short sugar-inducible promoter derived from a sweet potato sporamin gene (Spomin) that showed high-level expression of LUC under non-inducing condition. Activator of Spomin::LUC1 (ASML1) gene located downstream of the enhancer encoded an APETALA2 (AP2)-type AP2 domain protein, and this gene was recently shown to be responsible for the wrinkled1 mutation which causes defective accumulation of seed storage oil. Overexpression of ASML1 cDNA in sGsL plant resulted in enhanced expression of not only the LUC reporter but also endogenous sugar-inducible genes including At{beta}-Amy encoding {beta}-amylase. Transient co-expression of 35S::ASML1 with Spomin::LUC or At{beta}-Amy::LUC reporters in protoplasts resulted in an approximately 10-fold transactivation of LUC expression. This transactivation was lost when the C-terminal acidic region of ASML1 was deleted. Expression of ASML1 was high in reproductive organs, and ASML1 mRNA showed transient accumulation in leaves after treatment with 6% sucrose, whereas it did not respond to ABA. These results suggest that ASML1/WRI1 is a transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds.

Keywords: AP2-domain transcriptional activator; Arabidopsis; ASML1; Seed oil; Sugar-signaling; WRI1.
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