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Plant and Cell Physiology Advance Access published online on February 2, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci064
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received August 19, 2004
Accepted January 23, 2005

Short Communication

Isolation and Characterization of Cdnas Encoding An Enzyme with Glucosyltransferase Activity forcyclo-Dopa from Four O'clocks and Feather Cockscombs

Nobuhiro Sasaki 1, Katsuhiro Wada 2, Takatoshi Koda 2, Kichiji Kasahara 3, Taiji Adachi 4, and Yoshihiro Ozeki 1*

1 Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan
2 San-Ei Gen F.F.I., Inc., 1-1-11 Sanwa-machi, Toyonaka, Osaka 561-8588, Japan
3 1128 Moro-oka, Kohoku-ku, Yokohama, Kanagawa 222-0002, Japan
4 Department of Plant Science, College of Agriculture, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan

* To whom correspondence should be addressed.
Yoshihiro Ozeki, E-mail: ozeky{at}cc.tuat.ac.jp


  Abstract

cDNAs encoding an enzyme with UDP-glucose:cyclo-DOPA 5-O-glucosyltransferase activity were isolated from four o'clocks and feather cockscombs. Phylogenetic analysis of the amino acid sequences deduced from the cDNAs show that they represent a single subclade distinct from those of other phenylpropanoid and flavonoid glucosyltransferases. Changes in the amount of transcripts of the cDNA in four o'clocks correlated with the accumulation of betanin during flower development. The cDNAs isolated here were candidates for the gene of the enzyme involved in another pathway of betacyanin biosynthesis via glucosylation at the cyclo-DOPA step rather than at the betanidin step.

Keywords: betacyanin; Celosia cristata; cyclo-DOPA; glucosyltransferase; Mirabilis jalapa.
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N. Sasaki, Y. Abe, Y. Goda, T. Adachi, K. Kasahara, and Y. Ozeki
Detection of DOPA 4,5-Dioxygenase (DOD) Activity Using Recombinant Protein Prepared from Escherichia coli Cells Harboring cDNA Encoding DOD from Mirabilis jalapa
Plant Cell Physiol., May 1, 2009; 50(5): 1012 - 1016.
[Abstract] [Full Text] [PDF]


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