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Plant and Cell Physiology Advance Access published online on February 2, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci058
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received August 24, 2004
Accepted January 15, 2005

Regular Paper

Tuber-Specific Cytosolic Expression of a Bacterial Phosphoglucomutase in Potato (Solanum tuberosum L.) Dramatically Alters Carbon Partitioning

Anna Lytovchenko 1, Nicolas Schauer 1, Lothar Willmitzer 1, and Alisdair R. Fernie 1*

1 Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Golm, Germany

* To whom correspondence should be addressed.
Alisdair R. Fernie, E-mail: fernie{at}mpimp-golm.mpg.de


  Abstract

Constitutive antisense inhibition of the cytosolic isoform of phosphoglucomutase in the potato (Solanum tuberosum L.) results in restriction of photosynthesis, growth inhibition and modified tuber morphology and a severe restriction of tuber starch synthesis (Fernie et al. (2002) Planta 214: 510-520).Here we describe the consequences of the tuber-specific expression of an Escherichia coli phosphoglucomutase in the cytosol. Analysis of [14C]glucose metabolism by tuber discs isolated from wild type and transformants revealed that the rates of sucrose and starch synthesis were unaltered but that the rate of glycolysis was depressed in the transgenics. The transformant tubers also contained dramatically reduced amino acid content and significantly higher levels of ADP, but were characterised by elevated levels of Krebs cycle intermediates and an unaltered rate of respiration. In addition to these metabolic consequences of the overexpression of the E. coli enzyme we observed morphological changes in tubers with the transformants having a smaller number of larger tubers which exhibited delayed rates of sprouting with respect to the wild type. These results are discussed with respect to current models of the regulation of central plant metabolism and tuber dormancy.

Keywords: amino acids; carbohydrate metabolism; phosphoglucomutase; glycolysis; Solanum tuberosum L.; starch synthesis.
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