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Plant and Cell Physiology Advance Access published online on February 2, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci055
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received June 9, 2004
Revised January 7, 2005
Accepted January 13, 2005

Regular Paper

Decision of Spindle Poles and Division Plane by Double Preprophase Bands in a BY-2 Cell Line Expressing GFP-Tubulin

Arata Yoneda 1*, Minori Akatsuka 1, Hidemasa Hoshino 1, Fumi Kumagai 1, and Seiichiro Hasezawa 1

1 Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha 5-1-5, Kashiwa, Chiba Prefecture 277-8562 Japan

* To whom correspondence should be addressed.
Arata Yoneda, E-mail: yoneda{at}mbi.nifty.com


  Abstract

The preprophase band (PPB) of microtubules (MTs) is thought to be involved in deciding the future division site. In this study, we investigated the effects of double PPBs on spindle formation and the directional decision of cytokinesis by using transgenic BY-2 cells expressing GFP-tubulin. At prophase, most of the cells with double PPBs formed multipolar spindles, whereas all cells with single PPBs formed normal bipolar spindles; clearly implicating the PPB in deciding the spindle poles. At metaphase, however, both cell types possessed the bipolar spindles, indicating the existence of correctional mechanism(s) at prometaphase. From prometaphase to metaphase, the spindles in double PPB cells altered their directions to become oblique to the cell-elongating axis, and these orientations were maintained in the phragmoplast and resulted in the oblique division planes. These oblique cell plates decreased when actin microfilaments (MFs) were disrupted, and double actin-depleted zones (ADZs) appeared where the double PPBs had existed. These results suggest that the information necessary for proper cytokinesis may be transferred from the PPBs to the ADZs, even in the case of the double PPBs.

Keywords: Actin-depleted zone (ADZ); BY-GT16 cells; cytokinesis; double preprophase bands; microtubule; multipolar spindle.
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