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Plant and Cell Physiology Advance Access published online on February 2, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci053
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received October 29, 2004
Revised December 25, 2004
Accepted January 11, 2005

Regular Paper

Characterization of the Arabidopsis thaliana Mutant pcb2 Which Accumulates Divinyl Chlorophylls

Hiromitsu Nakanishi 1, Hatsumi Nozue 1, Kenji Suzuki 1, Yasuko Kaneko 2, Goro Taguchi 1, and Nobuaki Hayashida 1

1 Division of Gene Research, Department of Life Science, Research Center for Human and Environmental Sciences, Shinshu University, Ueda, 386-8567 Japan
2 Department of Regulation Biology, Faculty of Science, Saitama University, Saitama, 338-8570 Japan


  Abstract

We characterized the pcb2 (pale-green and chlorophyll b reduced 2) mutant. We found through electron microscopic observation that chloroplasts of pcb2 mesophyll cells lacked distinctive grana stacks. HPLC analysis showed that the pcb2 mutant accumulated divinyl chlorophylls, and the relative amount of divinyl chlorophyll b was remarkably less than divinyl chlorophyll a. The responsible gene was mapped in an area of 190 kb length at the upper arm of the 5th chromosome, and comparison of DNA sequences revealed a single nucleotide substitution causing a nonsense mutation in At5g18660. Complementation analysis confirmed that the wild-type of this gene suppressed the phenotypes of the mutation. Antisense transformants of the gene also accumulated divinyl chlorophylls. The genes homologous to At5g18660 are conserved in a broad range of species in the plant kingdom, and have similarity to reductases. Our results suggest that the PCB2 product is divinyl protochlorophyllide 8-vinyl reductase.

Keywords: Arabidopsis thaliana; chloroplast; complementation; divinyl protochlorophyllide 8-vinyl reductase; grana stack; pcb2 mutant.
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