Skip Navigation



Plant and Cell Physiology Advance Access published online on February 2, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci046
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
46/3/482    most recent
pci046v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Chang, M.
Right arrow Articles by Lee, H.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chang, M.
Right arrow Articles by Lee, H.-J.
Agricola
Right arrow Articles by Chang, M.
Right arrow Articles by Lee, H.-J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received October 26, 2004
Revised December 27, 2004
Accepted January 7, 2005

Short Communication

Cellular Internalization of Fluorescent Proteins Via Arginine-Rich Intracellular Delivery Peptide in Plant Cells

Microsugar Chang 1, Jyh-Ching Chou 2, and Han-Jung Lee 2*

1 Institute of Biotechnology and Department of Life Science, National Dong Hwa University, Hualien 974, Taiwan; Institute of Medical Sciences, Tzu Chi University, Hualien 970, Taiwan
2 Institute of Biotechnology and Department of Life Science, National Dong Hwa University, Hualien 974, Taiwan

* To whom correspondence should be addressed.
Han-Jung Lee, E-mail: hjlee{at}mail.ndhu.edu.tw


  Abstract

The protein delivery across cellular membranes or compartments is limited by low biomembrane permeability because of the hydrophobic characteristics of cell membranes. Usually the delivery processes utilize passive protein channels or active transporters to overcome the membrane impediment. In this report, we demonstrate that arginine-rich intracellular delivery (AID) peptide is capable of efficiently delivering fused fluorescent proteins unpreferentially into different plant tissues of both tomato (a dicot plant) and onion (a monocot plant) in a fully bioactive form. Thus, cellular internalization via AID peptide can be a powerful tool characterized by its simplicity, non-invasion, and high efficiency to express those bioactive proteins in planta or in plant cells in vivo. This novel method may alternatively provide broader applications of AID chimera in plants without the time-consuming transgenic approaches.

Keywords: Cellular internalization; Green fluorescent protein; Protein transduction; Recombinant protein; Transgenic plant.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
D. Dou, S. D. Kale, X. Wang, R. H.Y. Jiang, N. A. Bruce, F. D. Arredondo, X. Zhang, and B. M. Tyler
RXLR-Mediated Entry of Phytophthora sojae Effector Avr1b into Soybean Cells Does Not Require Pathogen-Encoded Machinery
PLANT CELL, July 1, 2008; 20(7): 1930 - 1947.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.