Plant and Cell Physiology Advance Access published online on February 2, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci044
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1 Agrobioteknologia eta Natura Baliabideetako Instituta, Nafarroako Unibertsitate Publikoa eta Consejo Superior de Investigaciones Científicas, Mutiloako etorbidea zenbaki gabe, 31192 Mutiloabeti, Nafarroa, Spain; University of Florida, IFAS, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL, 33850, USA
* To whom correspondence should be addressed. The capacity of plant heterotrophic organs to transport and accumulate incoming nutrients (mostly in the form of sucrose) directly impact their final size, crop productivity, and nutritional value. Endocytosis as a mechanism for nutrient uptake in heterotrophic cells was investigated using suspension culture cells of sycamore (Acer pseudoplatanus L.) and the endocytic inhibitors wortmannin and LY294002. Time-course analysis of sucrose uptake in intact walled cells revealed a two-phase process involving an initial 90 min wortmannin- and LY294002-insensitive sucrose uptake period, followed by a prolonged phase of rapid sucrose accumulation which was greatly inhibited by the two endocytic inhibitors. Walled cells were assessed for their capacity to incorporate the fluorescent endocytosis marker Lucifer Yellow-CH (LY) in the presence or absence of sucrose. Rates of sucrose and LY accumulation were virtually identical as was their response to wortmannin. In addition, LY incorporation increased as a function of external sucrose concentration. When sucrose was substituted by other sugars or amino acids, uptake of LY greatly diminished, indicating that sucrose itself is the primary signal of endocytosis. Microscopic observations revealed the formation of vesicles containing LY and its eventual accumulation on the vacuole when sucrose was present in the incubation medium. These results demonstrate the existence of a sucrose inducible endocytic process as a viable mechanism for solute transport into the vacuole of storage cells.
Received August 25, 2004
Revised December 26, 2004
Accepted January 7, 2005
Regular Paper
Sucrose Inducible Endocytosis As a Mechanism for Nutrient Uptake in Heterotrophic Plant Cells
2 Agrobioteknologia eta Natura Baliabideetako Instituta, Nafarroako Unibertsitate Publikoa eta Consejo Superior de Investigaciones Científicas, Mutiloako etorbidea zenbaki gabe, 31192 Mutiloabeti, Nafarroa, Spain
Ed Etxeberria, E-mail: eje{at}crec.ifas.ufl.edu
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