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Plant and Cell Physiology Advance Access published online on February 2, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci042
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received August 22, 2004
Revised December 24, 2004
Accepted January 7, 2005

Regular Paper

Metabolic Flux Analysis of the Phenylpropanoid Pathway in Elicitor-Treated Potato Tuber Tissue

Fumio Matsuda 1, Keiko Morino 1, Rieko Ano 2, Masaki Kuzawa 2, Kyo Wakasa 3, and Hisashi Miyagawa 1*

1 Division of Applied Life Sciences, Department of Agriculture, Kyoto University, Kyoto, 606-8502 Japan; Plant functions and their control, CREST, Japan Science and Technology Agency, Tokyo 103-0027, Japan
2 Division of Applied Life Sciences, Department of Agriculture, Kyoto University, Kyoto, 606-8502 Japan
3 Biotechnology Laboratory, National Institute of Crop Science, Tsukuba, 305-8515, Japan; Plant functions and their control, CREST, Japan Science and Technology Agency, Tokyo 103-0027, Japan

* To whom correspondence should be addressed.
Hisashi Miyagawa, E-mail: miyagawa{at}kais.kyoto-u.ac.jp


  Abstract

The effects of {beta}-1,3-oligosaccharide elicitor on the metabolism of phenylpropanoids in potato tuber were analyzed quantitatively, by monitoring the time-dependent changes in the levels of seven compounds. The elicitor treatment caused an increase in the pool size of octopamine and tyramine amides (N-p-coumaroyloctopamine, N-feruloyloctopamine,N-p-coumaroyltyramine, and N-feruloyltyramine), as well as a decrease in that of chlorogenic acid and putrescine amides (caffeoylputrescine and feruloylputrescine). An analysis of metabolic flux using stable isotope labeling and LC-MS detection clearly demonstrated that the changes in the pool size of these compounds were correlated with the changes in their flux for biosynthesis (Jin) upon the elicitor treatment. The increase in Jin in the cases of octopamine and tyramine amides was accompanied by an increase in flux for the transformation (Jout), indicating a rapid turnover of these compounds in the elicitor-treated tuber tissue. The result of the flux analysis indicated that the actual activation of the biosynthesis of octopamine and tyramine amides after the elicitor treatment was greater than that estimated from the changes in their levels in the potato tissue. These findings suggest that these amide compounds and their metabolic derivatives play an important role in the defense-related metabolism of phenylpropanoids in potato.

Keywords: hydroxycinnamic acid conjugates; metabolic flux analysis; phenylpropanoid pathway; plant defense response; Solanum tuberosum.
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