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Plant and Cell Physiology Advance Access published online on February 2, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci039
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received September 27, 2004
Revised December 19, 2004
Accepted December 22, 2004

Regular Paper

Expression of Randomly Integrated Single Complete Copy Transgenes Does Not Vary in Arabidopsis thaliana

Shingo Nagaya 1, Ko Kato 1*, Yuka Ninomiya 2, Rie Horie 2, Masami Sekine 1, Kazuya Yoshida 1, and Atsuhiko Shinmyo 1

1 Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630-0101, Japan
2 Research Association for Biotechnology, 2-3-9 Miyashita Bldg. Nishi-shinbashi, Minato-ku, Tokyo 105-0003, Japan

* To whom correspondence should be addressed.
Ko Kato, E-mail: kou{at}bs.naist.jp


  Abstract

The high variability of transgene expression is frequently observed in independent transgenic lines. Variability of transgene expression has been attributed to several factors, including differences in chromosome position, repeat sequences, and copy number. The eukaryotic genome, heterogeneous chromatin structure, is not homogeneous for transcriptional activity. Chromatin structure at the site of integration can affect transgene expression; this phenomenon is called the position effect. In this study, we investigated whether position effects confer variability of transgene expression in Arabidopsis thaliana. We analyzed the expression of randomly integrated single 'complete' (intact, non-truncated, non-rearranged) copy transgenes in Arabidopsis thaliana. Ten independent lines containing single complete copies of the transgene located at different chromosome positions showed very similar levels of transgene expression, and variability of transgene expression was not observed. This result indicates that position effects may not generally be a major cause of variability of transgene expression in Arabidopsis thaliana.

Keywords: Arabidopsis thaliana; chromatin; homology-dependent gene silencing (HDGS); position effect; post-transcriptional gene silencing (PTGS); single copy; transgene expression.
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