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Plant and Cell Physiology Advance Access published online on February 2, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci036
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received August 25, 2004
Accepted December 8, 2004

Short Communication

Study of the Constitutively Active Form of the {alpha} Subunit of Rice Heterotrimeric G Proteins

Katsuyuki Oki 1, Yukiko Fujisawa 1, Hisaharu Kato 1, and Yukimoto Iwasaki 1*

1 Fukui Prefectural University, Faculty of Bioscience, Kenjyojima, Matsuoka-cho, Yoshida-gun, Fukui 910-1195, Japan

* To whom correspondence should be addressed.
Yukimoto Iwasaki, E-mail: iwasaki{at}fpu.ac.jp


  Abstract

We used site-directed mutagenesis to engineer two constitutively active forms of the {alpha} subunit of a rice heterotrimeric G protein. The recombinant proteins produced from these novel cDNAs had GTP-binding activity but no GTPase activity. A chimeric gene for a constitutively active form of the {alpha}subunit was introduced into the rice mutant d1, which is defective for the {alpha}-subunit gene. All the transformants essentially showed a wild type phenotype compared to normal cultivars, although seed sizes were substantially increased and internode lengths also showed some increase.

Keywords: Constitutively active form; Heterotrimeric G protein; Rice; Site directed mutagenesis; Transgenic plant.
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[Abstract] [Full Text] [PDF]


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