Skip Navigation



Plant and Cell Physiology Advance Access published online on February 2, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci031
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
46/2/300    most recent
pci031v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kagaya, Y.
Right arrow Articles by Hattori, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kagaya, Y.
Right arrow Articles by Hattori, T.
Agricola
Right arrow Articles by Kagaya, Y.
Right arrow Articles by Hattori, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received June 10, 2004
Accepted November 24, 2004

Regular Paper

Indirect ABA-Dependent Regulation of Seed Storage Protein Genes by FUSCA3 Transcription Factor in Arabidopsis

Yasuaki Kagaya 1, Rie Okuda 1, Atsushi Ban 1, Ryoko Toyoshima 1, Kumiko Tsutsumida 1, Haruko Usui 2, Akiko Yamamoto 2, and Tsukaho Hattori 2*

1 Life Science Research Center, Mie University, Tsu 514-8507, Japan
2 Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464- 8601, Japan

* To whom correspondence should be addressed.
Tsukaho Hattori, E-mail: hattori{at}agr.nagoya-u.ac.jp


  Abstract

The key transcription factors that control seed maturation, ABSCISIC ACID INSENSEITIVE3 (ABI3) and FUSCA3 (FUS3), share homologous DNA-binding domains. Regulation of seed storage protein genes At2S3 and CRC by ABI3 and FUS3 was investigated using transgenic plants in which ABI3 and FUS3 could be ectopically induced by steroid hormones. Like ABI3, the presence of FUS3 led to expression of At2S3 and CRC in vegetative tissues. FUS3-mediated induction of CRC was completely dependent on exogenous ABA, while At2S3 was weakly induced without ABA but strongly enhanced with ABA. This ABA-dependency of FUS3-induced CRC and At2S3 expression was similar to that observed for ABI3. However, kinetic analysis revealed distinctions between the mechanisms of ABA- dependent CRC regulation by FUS3 or ABI3, and between target genes. While At2S3 activation by FUS3 was rapid, CRC induction by FUS3 in the presence of ABA, and by ABA followed in the presence of FUS3, took a significantly longer time (24-36 h). This suggested the involvement of an indirect mechanism requiring the ABA- and FUS3-dependent synthesis of intermediate regulatory factor(s). A chimeric protein composed of the FUS3 B3 domain, and a heterologous activation domain and nuclear localization signal exhibited a tight coupling with ABA regulation as observed for wild type FUS3. Simultaneous induction of FUS3 and ABI3 did not result in the synergistic activation of CRC and At2S3. Based on these results, similarities and differences in the mechanisms of seed storage protein gene regulation by FUS3 and ABI3 are discussed.

Keywords: ABI3; Arabidopsis thaliana; LEC1; cruciferin; 2S albumin; seed development.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol PlantHome page
H. Zhang and J. Ogas
An Epigenetic Perspective on Developmental Regulation of Seed Genes
Mol Plant, July 1, 2009; 2(4): 610 - 627.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M.-J. Gao, D. J. Lydiate, X. Li, H. Lui, B. Gjetvaj, D. D. Hegedus, and K. Rozwadowski
Repression of Seed Maturation Genes by a Trihelix Transcriptional Repressor in Arabidopsis Seedlings
PLANT CELL, January 1, 2009; 21(1): 54 - 71.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Mu, H. Tan, Q. Zheng, F. Fu, Y. Liang, J. Zhang, X. Yang, T. Wang, K. Chong, X.-J. Wang, et al.
LEAFY COTYLEDON1 Is a Key Regulator of Fatty Acid Biosynthesis in Arabidopsis
Plant Physiology, October 1, 2008; 148(2): 1042 - 1054.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J. Verdier and R. D. Thompson
Transcriptional Regulation of Storage Protein Synthesis During Dicotyledon Seed Filling
Plant Cell Physiol., September 1, 2008; 49(9): 1263 - 1271.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. Tang, A. Hou, M. Babu, V. Nguyen, L. Hurtado, Q. Lu, J. C. Reyes, A. Wang, W. A. Keller, J. J. Harada, et al.
The Arabidopsis BRAHMA Chromatin-Remodeling ATPase Is Involved in Repression of Seed Maturation Genes in Leaves
Plant Physiology, July 1, 2008; 147(3): 1143 - 1157.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Kotak, E. Vierling, H. Baumlein, and P. v. Koskull-Doring
A Novel Transcriptional Cascade Regulating Expression of Heat Stress Proteins during Seed Development of Arabidopsis
PLANT CELL, January 1, 2007; 19(1): 182 - 195.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. A. Braybrook, S. L. Stone, S. Park, A. Q. Bui, B. H. Le, R. L. Fischer, R. B. Goldberg, and J. J. Harada
Genes directly regulated by LEAFY COTYLEDON2 provide insight into the control of embryo maturation and somatic embryogenesis
PNAS, February 28, 2006; 103(9): 3468 - 3473.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Kagaya, R. Toyoshima, R. Okuda, H. Usui, A. Yamamoto, and T. Hattori
LEAFY COTYLEDON1 Controls Seed Storage Protein Genes through Its Regulation of FUSCA3 and ABSCISIC ACID INSENSITIVE3
Plant Cell Physiol., March 1, 2005; 46(3): 399 - 406.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.