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Plant and Cell Physiology Advance Access published online on February 2, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci025
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received September 17, 2004
Accepted November 28, 2004

Regular Paper

The Regulatory Networks of Gene Expression During the Sexual Differentiation of Chlamydomonas reinhardtii, as Analyzed by Mutants for Gametogenesis

Jun Abe 1, Takeaki Kubo 1, Tatsuaki Saito 2, and Yoshihiro Matsuda 3*

1 Department of Molecular Science, Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe 657-8501, Japan
2 Department of Applied Science, Faculty of Science, Okayama University of Science, Okayama 700-0005, Japan
3 Department of Biology, Faculty of Science, Kobe University, Nada-ku, Kobe 657-8501, Japan

* To whom correspondence should be addressed.
Yoshihiro Matsuda, E-mail: matsuday{at}kobe-u.ac.jp


  Abstract

Cells of Chlamydomonas reinhardtii differentiate into gametes under conditions of nitrogen (N) starvation, expressing the genes for the N-adaptation program and the gamete program. To investigate the regulatory networks of transcription among the N-starvation inducible genes, we examined the gene expression in dif mutants, affecting gametic differentiation. In a conditional mutant, dif2, the cells remained "vegetative"at the restrictive temperature and the induction of 20 out of 21 genes related to the two programs was impaired. They were expressed soon after transfer the cells to the permissive temperature, in parallel with the acquisition of mating ability. In an unconditional mutant, dif3, the cells could not differentiate into gametes at all, but the induction of only four genes (FUS1, NSG3, NSG6, NSG7) related to the gamete program was impaired. The results suggest that Dif3 regulates putative N-starvation signal transduction pathways at the downstream of a master regulator, Dif2. We also examined a light-dependent laboratory strain that was unable to become gametes in the dark. The "pregametes"placed in the dark, however, could induce normally all of the 21 genes, suggesting that light is required for the gametic differentiation at the translational and/or post-translational levels.

Keywords: Chlamydomonas reinhardtii; Nitrogen starvation inducible genes; Sexual differentiation mutants; Transcription regulation.
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