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Plant and Cell Physiology Advance Access published online on February 2, 2005
Plant and Cell Physiology, doi:10.1093/pcp/pci023
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Plant and Cell Physiology 2005 © The Japanese Society of Plant Physiologists (JSPP); all rights reserved.
Received August 30, 2004
Accepted November 18, 2004

Short Communication

Phosphorylation of Sphingoid Long-Chain Bases in Arabidopsis: Functional Characterization and Expression of the First Sphingoid Long-Chain Base Kinase Gene in Plants

Hiroyuki Imai 1* and Hideki Nishiura 1

1 Department of Biology, Graduate School of Natural Science, Konan University, Kobe 658-8501, Japan

* To whom correspondence should be addressed.
Hiroyuki Imai, E-mail: imai{at}konan-u.ac.jp


  Abstract

Sphingoid long-chain base (LCB) kinase catalyzes the phosphorylation of LCBs to form LCB 1-phosphates. Based on sequence identity to murine sphingosine kinase (mSPHK1), we cloned and characterized the first plant LCB kinase gene from Arabidopsis (AtLCBK1). Using recombinant AtLCBK1 protein fromEscherichia coli cells, we confirmed that the enzyme specifically phosphorylated D-erythro-dihydrosphingosine (DHS), but not N-acetyl-DHS or D-threo-DHS. AtLCBK1 also phosphorylated D-erythro-sphingosine, trans-4, trans-8-sphingadienine and phytosphingosine. We found that AtLCBK1 mRNA is expressed highly in flowers. AtLCBK1 transcripts were slightly increased by low-humidity or abscisic acid treatments, suggesting that AtLCBK1 is constitutively expressed under these treatments.

Keywords: ABA; dehydration stress; long-chain base; phytosphingosine; sphingosine kinase; T87 cells.
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