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Plant and Cell Physiology Advance Access published online on November 22, 2004
Plant and Cell Physiology, doi:10.1093/pcp/pch193
© 2004 by The Japanese Society of Plant Physiologists
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Received May 17, 2004
Accepted September 9, 2004

Regular Paper

Azetidine Induced Accumulation of Class I Small Heat Shock Proteins in the Soluble Fraction Provide Thermotolerance in Soybean Seedlings

Tsung-Luo Jinn 1, Chi-Chou Chiu 1, Wei-Wen Song 1, Yi-Ming Chen 1, and Chu-Yung Lin 1*

1 Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan, ROC

* To whom correspondence should be addressed.
Chu-Yung Lin, E-mail: chuyung{at}ccms.ntu.edu.tw


  Abstract

Accumulation of class I small heat shock proteins (sHSPs) is induced by the proline analog, azetidine-2-carboxylic acid (Aze) in soybean seedlings. Class I sHSPs induced by Aze reached the same levels as induced by heat shock at 40°C. In seedlings that were challenged with a non-permissive temperature at 45°C, only the 10 mM Aze (6 h) -> 10 mM Pro (24 h) treatment conferred thermotolerance as assessed by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining. The purified class I sHSPs induced by Aze were functional in vitro and protected proteins from thermal denaturation in a chaperone activity assay. Amino acid composition analysis indicated Aze was not incorporated into de novo synthesized class I sHSPs. The accumulation of class I sHSPs recovered in the soluble postribosomal supernatant fraction was important for acquisition of thermotolerance. We suggest that both the accumulation of class I sHSPs as well as their presence in soluble fraction are important for establishment of thermotolerance.

Keywords: amino acid analog; azetidine; Glycine max; heat shock; plant class I sHSPs; thermotolerance.
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