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Plant and Cell Physiology Advance Access originally published online on August 10, 2009
Plant and Cell Physiology 2009 50(9):1674-1680; doi:10.1093/pcp/pcp112
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Is the Photosystem II Complex a Monomer or a Dimer?

Mai Watanabe1, Masako Iwai2, Rei Narikawa1 and Masahiko Ikeuchi1,*

1Department of Life Sciences (Biology), Graduate School of Arts and Science, University of Tokyo, Komaba, Meguro, Tokyo, 153-8902 Japan
2Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Yamasaki, Noda, Chiba, 278-8510 Japan

*Corresponding author: E-mail, mikeuchi{at}bio.c.u-tokyo.ac.jp; Fax, +81-3-5454-4337.


  Abstract

It is widely believed that the photosystem II (PSII) complex may function as a dimer in the thylakoid membrane. Here, we report experimental conversion from the monomeric PSII to the dimeric form by treatment with high concentrations of n-dodecyl-β-D-maltopyranoside (DM). The content of the PSII monomer in a PsbTc deletion mutant was much higher than in the wild type when solubilized with low concentrations of DM. However, upon treatment with higher concentrations of DM, the PSII dimer was also recovered in the PsbTc deletion mutant. These results suggest that there are at least two distinct processes of dimerization: (i) PsbTc dependent and (ii) DM induced. We discuss the results with regard to the native assembly form(s) of PSII.

Keywords: Blue-native PAGE - Dimer - Monomer - PSII - psbM - psbTc

Abbreviations: BN, Blue-native; DM, n-dodecyl-β-D-maltopyranoside.

(Received May 25, 2009; Accepted July 29, 2009)
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