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Plant and Cell Physiology Advance Access originally published online on March 12, 2009
Plant and Cell Physiology 2009 50(4):904-908; doi:10.1093/pcp/pcp042
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

This article appears in the following Plant and Cell Physiology issue: Special Issue Articles: Photosynthesis [View the issue table of contents]

Short Communication

Visualization of Plastids in Pollen Grains: Involvement of FtsZ1 in Pollen Plastid Division

Lay Yin Tang1, Noriko Nagata2, Ryo Matsushima1, Yuling Chen3,4, Yasushi Yoshioka3 and Wataru Sakamoto1,*

1Research Institute for Bioresources, Okayama University, Kurashiki, Okayama, 710-0046 Japan
2Department of Chemical and Biological Sciences, Japan Women's University, Bunkyo-ku, Tokyo, 112-8681 Japan
3Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya, 464-8602 Japan

*Corresponding author: E-mail, saka{at}rib.okayama-u.ac.jp; Fax, +81-86-434-1206.


  Abstract

Visualizing organelles in living cells is a powerful method to analyze their intrinsic mechanisms. Easy observation of chlorophyll facilitates the study of the underlying mechanisms in chloroplasts, but not in other plastid types. Here, we constructed a transgenic plant enabling visualization of plastids in pollen grains. Combination of a plastid-targeted fluorescent protein with a pollen-specific promoter allowed us to observe the precise number, size and morphology of plastids in pollen grains of the wild type and the ftsZ1 mutant, whose responsible gene plays a central role in chloroplast division. The transgenic material presented in this work is useful for studying the division mechanism of pollen plastids.

Keywords: FtsZ1 - Fluorescent protein - Male gametophyte - Plastid division - Pollen

Abbreviations: ARC, accumulation and replication of chloroplasts; GFP, green fluorescent protein; IF2, translation initiation factor 2; PD, plastid-dividing; RFP, red fluorescent protein; VC, vegetative cell.

4Present address: College of Life Science, Hebei Normal University, Shijiazhuang 050016, PR China.

(Received January 29, 2009; Accepted March 4, 2009)
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