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Plant and Cell Physiology Advance Access originally published online on March 3, 2009
Plant and Cell Physiology 2009 50(4):869-878; doi:10.1093/pcp/pcp037
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

This article appears in the following Plant and Cell Physiology issue: Special Issue Articles: Photosynthesis [View the issue table of contents]

Cold Shock Domain Proteins Affect Seed Germination and Growth of Arabidopsis thaliana Under Abiotic Stress Conditions

Su Jung Park, Kyung Jin Kwak, Tae Rin Oh, Yeon Ok Kim and Hunseung Kang*

Department of Plant Biotechnology, Agricultural Plant Stress Research Center and Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 500-757, Korea

*Corresponding author: E-mail, hskang{at}jnu.ac.kr; Fax, +82-62-530-2047.


  Abstract

Unlike the well-known functions of cold shock proteins in prokaryotes during cold adaptation, the biological functions of cold shock domain proteins (CSDPs) in plants remain largely unknown. Here, we examined the functional roles of two structurally different CSDPs, CSDP1 harboring a long C-terminal glycine-rich region interspersed with seven CCHC-type zinc fingers and CSDP2 containing a far shorter glycine-rich region interspersed with two CCHC-type zinc fingers, in Arabidopsis thaliana under stress conditions. CSDP1 overexpression delayed the seed germination of Arabidopsis under dehydration or salt stress conditions, whereas CSDP2 overexpression accelerated the seed germination of Arabidopsis under salt stress conditions. CSDP1 and CSDP2 rescued the cold-sensitive glycine-rich RNA-binding protein 7 mutant plants from freezing damage to a different degree, and this rescuing capability was correlated with their ability to complement the cold-sensitive Escherichia coli BX04 mutant at low temperatures. The nucleic acid-binding properties of CSDPs varied depending on the N-terminal cold shock domain and the C-terminal glycine-rich zinc finger region. Collectively, these results showed that CSDP1 and CSDP2 perform different functions in seed germination and growth of Arabidopsis under stress conditions, and that the glycine-rich region interspersed with CCHC-type zinc fingers is particularly important for its nucleic acid-binding activities and function.

Keywords: Abiotic stress - Arabidopsis thaliana - Cold shock domain protein - Cold shock protein - RNA chaperone

Abbreviations: CaMV, cauliflower mosaic virus; CSD, cold shock domain; CSDP, cold shock domain protein; CSP, cold shock protein; GRP7, glycine-rich RNA-binding protein 7; RT–PCR, reverse transcripion–PCR.

(Received February 2, 2009; Accepted February 26, 2009)
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