Skip Navigation


Plant and Cell Physiology Advance Access originally published online on February 13, 2009
Plant and Cell Physiology 2009 50(4):801-811; doi:10.1093/pcp/pcp024
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
50/4/801    most recent
pcp024v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hamada, T.
Right arrow Articles by Sonobe, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hamada, T.
Right arrow Articles by Sonobe, S.
Agricola
Right arrow Articles by Hamada, T.
Right arrow Articles by Sonobe, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

This article appears in the following Plant and Cell Physiology issue: Special Issue Articles: Photosynthesis [View the issue table of contents]

The Putative RNA-Processing Protein, THO2, is a Microtubule-Associated Protein in Tobacco

Takahiro Hamada1,2,4,*, Hisako Igarashi1,3,4, Ryoichi Taguchi1, Masayuki Fujiwara2, Yoichiro Fukao2, Teruo Shimmen1, Etsuo Yokota1 and Seiji Sonobe1

1Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo, 678-1297 Japan
2Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, 630-0101 Japan
3National Institute for Basic Biology, Myodaiji-cho, Okazaki, Aichi, 444-8585 Japan

*Corresponding author: E-mail, hama{at}bs.naist.jp; Fax, +81-743-72-5529.


  Abstract

THO2 is a component of the THO–TREX (transcription and export factor) complex that participates in mRNA metabolism and export from the nucleus in yeast and animal cells. Here we report that tobacco putative THO2-related protein (NtTHO2) is a microtubule-associated protein, which directly binds to microtubules in vitro and co-localizes with cortical microtubules in vivo. We purified endogenous NtTHO2 by cycles of microtubule polymerization–depolymerization from crude extracts of tobacco BY-2 miniprotoplasts. Purified NtTHO2 sedimented with microtubules in vitro. Immunofluore-scence revealed that NtTHO2 was present in both the nucleus and cytoplasm. In interphase, cytoplasmic NtTHO2 was localized along cortical microtubules. In the mitotic phase, NtTHO2 was localized to the mitotic spindle but not to either the preprophase band or the phragmoplast. In mature cells of seedling roots, and in BY-2 cells in which proliferation was stopped by removing 2,4-D, NtTHO2 staining was confined mainly to the nucleolus. These results suggest that NtTHO2 is a multifunctional protein that participates in mRNA metabolism, and also functions within the cortical microtubules and mitotic spindle.

Keywords: BY-2 cells - Microtubule - Microtubule-associated protein - Nicotiana tabacum - Nucleolus - THO2

Abbreviations: BSA, bovine serum albumin; DTT, dithio-threitol; LC, liquid chromatography; MAP, microtubule-associated protein; MS, mass spectrometry; MTP, microtubule protein; PBS, phosphate-buffered saline; PMSF, phenylmethylsulfonyl fluoride; RNP, ribonucleoprotein; TREX, transcription and export factor.

4These authors contributed equally to this work.

(Received December 16, 2008; Accepted February 10, 2009)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.