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Plant and Cell Physiology Advance Access originally published online on January 2, 2009
Plant and Cell Physiology 2009 50(2):423-428; doi:10.1093/pcp/pcn206
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Increase in Ascorbate Content of Transgenic Tobacco Plants Overexpressing the Acerola (Malpighia glabra) Phosphomannomutase Gene

Adebanjo A. Badejo, Hani A. Eltelib, Kazunari Fukunaga, Yukichi Fujikawa and Muneharu Esaka*

Graduate School of Biosphere Sciences, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8528 Japan

*Corresponding author: E-mail, mesaka{at}hiroshima-u.ac.jp; Fax, +81-82-424-7916.


  Abstract

Phosphomannomutase (PMM; EC 5.4.2.8 [EC] ) catalyzes the interconversion of mannose-6-phosphate to mannose-1-phosphate in the Smirnoff–Wheeler pathway for the biosynthesis of L-ascorbic acid (AsA). We have cloned the PMM cDNA from acerola (Malpighia glabra), a plant containing an enormous amount of AsA. The AsA contents correlate with the PMM gene expression of the ripening fruits and leaves. The PMM activities in the leaves of acerola, tomato and Arabidopsis correlate with their respective AsA contents. Transgenic tobacco plants overexpressing the acerola PMM gene showed about a 2-fold increase in AsA contents compared with the wild type, with a corresponding correlation with the PMM transcript levels and activities.

Keywords: Acerola (Malpighia glabra) - Ascorbic acid - Enzyme activity - Phosphomannomutase - Phosphomannose isomerase - Transgenic tobacco

Abbreviations: AsA, L-ascorbic acid; GMP, GDP-D-mannose pyrophosphorylase; G6PDH, glucose 6-phosphate dehydro-genase; MgPMM, Malpighia glabra PMM; PGI, phosphoglucose isomerase; PMI, phosphomannose isomerase; PMM, phosphomannomutase; RT–PCR, reverse transcription–PCR; SW, Smirnoff–Wheeler.

(Received November 17, 2008; Accepted December 23, 2008)
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