Skip Navigation


Plant and Cell Physiology Advance Access originally published online on January 12, 2009
Plant and Cell Physiology 2009 50(2):413-422; doi:10.1093/pcp/pcp003
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
50/2/413    most recent
pcp003v2
pcp003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Kurasawa, K.
Right arrow Articles by Nishitani, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kurasawa, K.
Right arrow Articles by Nishitani, K.
Agricola
Right arrow Articles by Kurasawa, K.
Right arrow Articles by Nishitani, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Japanese Society of Plant Physiologists are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

The AtXTH28 Gene, a Xyloglucan Endotransglucosylase/Hydrolase, is Involved in Automatic Self-Pollination in Arabidopsis thaliana

Kasumi Kurasawa1, Akihiro Matsui1,5, Ryusuke Yokoyama1, Tomoko Kuriyama2, Takeshi Yoshizumi2, Minami Matsui2, Keita Suwabe3, Masao Watanabe3,4 and Kazuhiko Nishitani1,*

1Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Sendai, 980-8578 Japan
2Plant Functional Genomics Research Team, Plant Functional Genomics Research Group, Plant Science Center, RIKEN Yokohama Institute, Tsurumi-ku, Yokohama, Kanagawa, 230-0045 Japan
3Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan
4The 21st Century Center of Excellence Program, Iwate University, Morioka, 020-8550 Japan

*Corresponding author: E-mail, nishitan{at}m.tains.tohoku.ac.jp; Fax, +81-22-795-6669.


  Abstract

Successful automatic self-pollination in flowering plants is dependent on the correct development of reproductive organs. In the stamen, the appropriate growth of the filament, which largely depends on the mechanical properties of the cell wall, is required to position the anther correctly close to the stigma at the pollination stage. Xyloglucan endotransglucosylase/hydrolases (XTHs) are a family of enzymes that mediate the construction and restructuring of xyloglucan cross-links, thereby controlling the extensibility or mechanical properties of the cell wall in a wide variety of plant tissues. Our reverse genetic analysis has revealed that a loss-of-function mutation of an Arabidopsis XTH family gene, AtXTH28, led to a decrease in capability for self-pollination, probably due to inhibition of stamen filament growth. Our results also suggest that the role of AtXTH28 in the development of the stamen is not functionally redundant with its closest paralog, AtXTH27. Thus, our finding indicates that AtXTH28 is specifically involved in the growth of stamen filaments, and is required for successful automatic self-pollination in certain flowers in Arabidopsis thaliana.

Keywords: Arabidopsis - Cell wall - Self-pollination - Stamen filament - XTH

Abbreviations: DIG, digoxigenin; GUS, β-glucuronidase; MS, Murashige and Skoog; RT–PCR, reverse transcription–PCR; SC, self-compatibility; SI, self-incompatibility; XTH, xyloglucan endotransglucosylase/hydrolase.

5Present address: Plant Genomic Network Research Team, Plant Functional Genomics Research Group, Plant Science Center, RIKEN Yokohama Institute, Tsurumi-ku, Yokohama, Kanagawa, 230-0045 Japan.

(Received November 26, 2008; Accepted January 4, 2009)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.