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Plant and Cell Physiology Advance Access originally published online on January 8, 2009
Plant and Cell Physiology 2009 50(2):374-382; doi:10.1093/pcp/pcn204
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© The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Phosphatidylglycerol Depletion Induces an Increase in Myxoxanthophyll Biosynthetic Activity in Synechocystis PCC6803 Cells

Ildiko Domonkos1, Przemyslaw Malec2, Hajnalka Laczko-Dobos1, Ozge Sozer1, Kinga Klodawska2, Hajime Wada3, Kazimierz Strzalka2 and Zoltan Gombos1,*

1Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, H-6701 Szeged, Hungary
2Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, PL-30-387 Krakow, Poland
3Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Tokyo, 153-8902 Japan

*Corresponding author: E-mail, gombos.zoltan{at}gmail.com; Fax, +36-62-433-434


  Abstract

Phosphatidylglycerol (PG) depletion suppressed the oxygen-evolving activity of Synechocystis PCC6803 pgsA mutant cells. Shortage of PG led to decreased photosynthetic activity, which, similar to the effect of high light exposure, is likely to generate the production of reactive oxygen species (ROS) or free radicals. Protection of the PG-depleted cells against light-induced damage increased the echinenone and myxoxanthophyll content of the cells. The increased carotenoid content was localized in a soluble fraction of the cells as well as in isolated thylakoid and cytoplasmic membranes. The soluble carotenoid fraction contained carotene derivatives, which may bind to proteins. These carotene–protein complexes are similar to orange carotenoid protein that is involved in yielding protection against free radicals and ROS. An increase in the content of myxoxanthophyll and echinenone upon PG depletion suggests that PG depletion regulates the biosynthetic pathway of specific carotenoids.

Keywords: Carotenoids - Myxoxanthophyll - PG depletion - Photosynthesis - Protection - SynechocystisPCC6803

Abbreviations: OCP, orange carotenoid protein; pBQ, 1,4-p-benzoquinone; PG, phosphatidylglycerol; PMSF, phenyl-methylsulfonyl fluoride; QA, primary quinone electron acceptor of PSII; QB, secondary quinone electron acceptor of PSII; ROS, reactive oxygen species

(Received October 30, 2008; Accepted December 24, 2008)
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