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Plant and Cell Physiology Advance Access originally published online on December 26, 2008
Plant and Cell Physiology 2009 50(2):191-202; doi:10.1093/pcp/pcn188
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Antisense Inhibition of the PsbX Protein Affects PSII Integrity in the Higher Plant Arabidopsis thaliana

José G. García-Cerdán1, Dmitry Sveshnikov1,2, David Dewez3, Stefan Jansson4, Christiane Funk1,4 and Wolfgang P. Schröder1,4,*

1Department of Chemistry, Umeå University, SE-901 87 Umeå, Sweden
2Potato Research Centre, Agriculture and Agri-Food Canada, PO Box 20280, Fredericton, NB, E3B 4Z7, Canada
3Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA
4Umeå Plant Science Centre (UPSC), Umeå University, SE-901 87 Umeå, Sweden

*Corresponding author: E-mail, Wolfgang.Schroder{at}chem.umu.se; Fax, +46 90 786 7661.


  Abstract

PSII, the oxygen-evolving complex of photosynthetic organisms, contains an intriguingly large number of low molecular weight proteins. PsbX, one of these proteins, is ubiquitous in PSII complexes of cyanobacteria and plants. In previous studies, deletion of the PsbX protein in cyanobacteria has not resulted in clear phenotypic changes. Here we report the construction of an antisense (AS-PsbX) line in Arabidopsis thaliana with <10% of wild-type PsbX levels. AS-PsbX plants are capable of photoautotrophic growth, but biochemical, biophysical and immunological evidence demonstrates that reduction of PsbX contents leads to reduced levels of functional assembled PSII core complexes, while the light-harvesting antennae are not affected. In addition, levels of phosphorylation of the core proteins D1, D2 and CP43 are severely reduced in the antisense plants relative to their wild-type counterparts. We conclude that PsbX is important for accumulation of functional PSII.

Keywords: Chloroplast - OJIP - Photosynthesis - PSII - Reaction centre - Thermoluminiscence - Thylakoid membrane

Abbreviations: FeCy, ferrocyanide; LHC, light-harvesting complex; NPQ, non-photochemical quenching; PEA, plant efficiency analyzer; PAM, pulse amplitude modulated; PpBQ, phenyl-p-benzoquinone; TL, thermoluminescence.

(Received September 22, 2008; Accepted November 27, 2008)
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