Skip Navigation


Plant and Cell Physiology Advance Access originally published online on November 17, 2008
Plant and Cell Physiology 2009 50(1):58-66; doi:10.1093/pcp/pcn168
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
50/1/58    most recent
pcn168v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Google Scholar
Right arrow Articles by Kato, Y.
Right arrow Articles by Fujiwara, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kato, Y.
Right arrow Articles by Fujiwara, T.
Agricola
Right arrow Articles by Kato, Y.
Right arrow Articles by Fujiwara, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Japanese Society of Plant Physiologists are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Special Issue - Regular Paper

Highly Boron Deficiency-Tolerant Plants Generated by Enhanced Expression of NIP5;1, a Boric Acid Channel

Yuichi Kato1,2,4, Kyoko Miwa2, Junpei Takano2,6, Motoko Wada1,5 and Toru Fujiwara2,3,*

1Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan
2Biotechnology Research Center, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan
3Solution-Oriented Research for Science and Technology (SORST), Japan Science and Technology Agency (JST), Japan

* Corresponding author: E-mail, atorufu{at}mail.ecc.u-tokyo.ac.jp; Fax, +81-3-5841-2408.


  Abstract

Boron (B) is an essential element for plants, and B deficiency is a worldwide agricultural problem. In B-deficient areas, B is often supplied as fertilizer, but excess B can be toxic to both plants and animals. Generation of B deficiency-tolerant plants could reduce B fertilizer use. Improved fertility under B-limiting conditions in Arabidopsis thaliana by overexpression of BOR1, a B transporter, has been reported, but the root growth was not improved by the BOR1 overexpression. In this study, we report that enhanced expression of NIP5;1, a boric acid channel for efficient B uptake, resulted in improved root elongation under B-limiting conditions in A. thaliana. An NIP5;1 activation tag line, which has a T-DNA insertion with enhancer sequences near the NIP5;1 gene, showed improved root elongation under B limitation. We generated a construct which mimics the tag line: the cauliflower mosaic virus 35S RNA promoter was inserted at 1,357 bp upstream of the NIP5;1 transcription initiation site. Introduction of this construct into the nip5;1-1 mutant and the BOR1 overexpresser resulted in enhanced expression of NIP5;1 and improved root elongation under low B supply. Furthermore, one of the transgenic lines exhibited improved fertility and short-term B uptake. Our results demonstrate successful improvement of B deficiency tolerance and the potential of enhancing expression of a mineral nutrient channel gene to improve growth under nutrient-limiting conditions.

Keywords: Arabidopsis thaliana - Boron - Channel - Deficiency tolerance - Nodulin 26-like intrinsic proteins - Transporter

Abbreviations: CaMV 35S promoter, cauliflower mosaic virus 35S RNA promoter; MIP, major intrinsic protein; NIP, nodulin 26-like intrinsic protein; PIP, plasma membrane intrinsic protein; RT–PCR, reverse transcription–PCR.

4Present address: Mizuho Bank, Shinbashi Branch, Shinbashi 2-1-3, Tokyo 105-0004, Japan

5Present address: Nippn Menard Cosmetic, Torimicho 2-7, Nagoya 451-0071, Japan

6Present address: Graduate School of Agriculture, Hokkaido University, Kita-9, Nishi-9, Sapporo 060-8589, Japan

(Received August 30, 2008; Accepted October 30, 2008)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Tree PhysiolHome page
R. K. Tewari, P. Kumar, and P. N. Sharma
Morphology and oxidative physiology of boron-deficient mulberry plants
Tree Physiol, November 20, 2009; (2009) tpp093v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Fujiwara and T. Matoh
Plant Nutrition--Roots of Life for Fundamental Biology and Better Crop Production
Plant Cell Physiol., January 1, 2009; 50(1): 2 - 4.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.