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Plant and Cell Physiology Advance Access originally published online on July 16, 2008
Plant and Cell Physiology 2008 49(9):1390-1395; doi:10.1093/pcp/pcn103
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

A Unique Caleosin in Oil Bodies of Lily Pollen

Pei-Luen Jiang1, Guang-Yuh Jauh2, Co-Shing Wang1 and Jason T.C. Tzen1,3,*

1 Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan
2 Institute of Plant and Microbial Biology, Academia Sinica, Taipei 115, Taiwan
3 Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan

*Corresponding author: E-mail, TCTZEN{at}dragon.nchu.edu.tw: Fax, +886-4-22853527.


  Abstract

In view of the recent isolation of stable oil bodies as well as a unique oleosin from lily pollen, this study examined whether other minor proteins were present in this lipid-storage organelle. Immunological cross-recognition using antibodies against three minor oil-body proteins from sesame suggested that a putative caleosin was specifically detected in the oil-body fraction of pollen extract. A cDNA fragment encoding this putative pollen caleosin, obtained by PCR cloning, was confirmed by immunodetection and MALDI-MS analyses of the recombinant protein over-expressed in Escherichia coli and the native form. Caleosin in lily pollen oil bodies seemed to be a unique isoform distinct from that in lily seed oil bodies.

Keywords: Caleosin - Lily (Lilium longiflorum Thunb.) - Oil bodies - Oleosin - Pollen

Abbreviations: PL(s), phospholipid(s); TAG(s), triacylglycerol(s).

(Received May 15, 2008; Accepted July 13, 2008)
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