Skip Navigation


Plant and Cell Physiology Advance Access originally published online on June 17, 2008
Plant and Cell Physiology 2008 49(8):1165-1175; doi:10.1093/pcp/pcn091
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
49/8/1165    most recent
pcn091v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Paschold, A.
Right arrow Articles by Baldwin, I. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Paschold, A.
Right arrow Articles by Baldwin, I. T.
Agricola
Right arrow Articles by Paschold, A.
Right arrow Articles by Baldwin, I. T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Jasmonate Perception Regulates Jasmonate Biosynthesis and JA-Ile Metabolism: The Case of COI1 in Nicotiana attenuata

Anja Paschold1, Gustavo Bonaventure, Merijn R. Kant and Ian T. Baldwin*

Max Planck Institute for Chemical Ecology, Department of Molecular Ecology, Hans-Knöll-Strasse 8, D-07745 Jena, Germany

*Corresponding author: E-mail, Baldwin{at}ice.mpg.de; Fax, +49-3641-571102.


  Abstract

CORONATINE INSENSITIVE 1 (COI1) is a well-known key player in processes downstream of jasmonic acid (JA) biosynthesis: silencing COI1 in Nicotiana attenuata (ir-coi1) makes plants insensitive to JA, prevents the up-regulation of JA-mediated defenses and decreases the plant's resistance to herbivores and pathogens. In agreement with previous studies, we observed that regulation of several JA biosynthesis genes elicited by Manduca sexta oral secretions (OS) is COI1 dependent. In response to wounding and application of OS ir-coi1 plants accumulate 75% less JA compared with wild-type plants (WT), resembling JA levels found in plants silenced in the key enzyme in JA biosynthesis LIPOXYGENASE 3 (as-lox). However, while OS-elicited as-lox plants also accumulated lower levels of the JA-conjugate JA–isoleucine (JA-Ile) than did WT plants, JA-Ile accumulation in ir-coi1 was higher, prolonged and peaked with a delay of 30 min. In vivo substrate feeding experiments of N. attenuata demonstrate that the increased and prolonged JA-Ile accumulation pattern in ir-coi1 is not the result of altered substrate availability, i.e. of JA and/or Ile, but is due to an ~6-fold decrease in JA-Ile turnover. These results provide the first evidence for a second, novel regulatory feedback function of COI1 in enhancing JA-Ile turnover. Hence, in addition to its control over JA biosynthesis, COI1 might fine-tune the dynamics of the jasmonate response after induction by herbivore elicitors.

Keywords: CORONATINE INSENSITIVE1 - Jasmonic acid - Jasmonic acid–isoleucine - Nicotiana attenuata - Solanum lycopersicum

Abbreviations: ACX1, ACYL-COENZYME A OXIDASE 1; ANOVA, analysis of variance; AOC, ALLENE OXIDE CYCLASE; AOS, ALLENE OXIDE SYNTHASE; as-lox, N. attenuata silenced in LOX3 via transformation with an antisense construct; COI1, CORONATINE INSENSITIVE 1; ir-coi1, N. attenuata silenced in COI1 via transformation with an inverted repeat construct; FDR, false discovery rate; JA, jasmonic acid; JA-Ile, jasmonic acid–isoleucine conjugate; JAR, JASMONIC ACID RESISTANT; LOX3, LIPOXYGENASE 3; MeJA, methyl jasmonate; OPDA, 12-oxophytodienoic acid; OPR3, OPDA REDUCTASE 3; OS, oral secretions collected from Manduca sexta larvae; 1RT–PCR, quantitative reverse transcription–PCR; TD, THREONINE DEAMINASE; WT, wild type.

1Present address: Centre for Plant Molecular Biology (ZMBP), Department of General Genetics, Eberhard-Karls-University, Auf der Morgenstelle 28, D-72076 Tübingen, Germany.

(Received April 13, 2008; Accepted June 8, 2008)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Dabrowska, D. Freitak, H. Vogel, D. G. Heckel, and W. Boland
The phytohormone precursor OPDA is isomerized in the insect gut by a single, specific glutathione transferase
PNAS, September 22, 2009; 106(38): 16304 - 16309.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.