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Plant and Cell Physiology Advance Access originally published online on April 3, 2008
Plant and Cell Physiology 2008 49(5):835-842; doi:10.1093/pcp/pcn058
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

CRR23/NdhL is a Subunit of the Chloroplast NAD(P)H Dehydrogenase Complex in Arabidopsis

Hideyuki Shimizu1, Lianwei Peng2, Fumiyoshi Myouga3, Reiko Motohashi4, Kazuo Shinozaki3 and Toshiharu Shikanai2,*

1Graduate School of Agriculture, Kyushu University, Higashi-ku, Fukuoka, 812-8581 Japan
2Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan
3Plant Genomic Network Research Team, RIKEN Plant Science Center, Tsurumi-ku, Yokohama, 203-0045 Japan
4Faculty of Agriculture, University of Shizuoka, Suruga-ku, Shizuoka, 422-8529 Japan

*Corresponding author: E-mail, shikanai{at}pmg.bot.kyoto-u.ac.jp; Fax, +81-75-753-4257.


  Abstract

The chloroplast NAD(P)H dehydrogenase (NDH) complex functions in PSI cyclic and chlororespiratory electron transport in higher plants. Eleven plastid-encoded and three nuclear-encoded subunits have been identified so far, but the entire subunit composition, especially of the putative electron donor-binding module, is unclear. We isolated Arabidopsis thaliana crr23 (chlororespiratory reduction) mutants lacking NDH activity according to the absence of a transient increase in Chl fluorescence after actinic light illumination. Although CRR23 shows similarity to the NdhL subunit of cyanobacterial NDH-1, it has three transmembrane domains rather than the two in cyanobacterial NdhL. Unlike cyanobacterial NdhL, CRR23 is essential for stabilizing the NDH complex, which in turn is required for the accumulation of CRR23. Furthermore, CRR23 and NdhH, a subunit of chloroplast NDH, co-localized in blue-native gel. All the results indicate that CRR23 is an ortholog of cyanobacterial ndhL in Arabidopsis, despite its diversity of structure and function.

Keywords: Arabidopsis - Chloroplast - Cyclic electron transport - NAD(P)H dehydrogenase - Photosynthesis

Abbreviations: AL, actinic light; BN, blue native; crr, chlororespiratory reduction; DM, dodecyl maltoside; ETR, electron transport rate; NDH, NAD(P)H dehydrogenase; NPQ, non-photochemical quenching; PAM, pulse amplitude-modulated; PFD, photon flux density; PQ, plastoquinone; RT–PCR, reverse transcription–PCR.

(Received February 29, 2008; Accepted April 1, 2008)
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