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Plant and Cell Physiology Advance Access originally published online on February 7, 2008
Plant and Cell Physiology 2008 49(3):396-410; doi:10.1093/pcp/pcn016
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Expression of Protein Complexes and Individual Proteins Upon Transition of Etioplasts to Chloroplasts in Pea (Pisum sativum)

Eira Kanervo1, Munna Singh2, Marjaana Suorsa1, Virpi Paakkarinen1, Eveliina Aro1, Natalia Battchikova1 and Eva-Mari Aro1,*

1Department of Biology, University of Turku, FIN-20014 Turku, Finland
2Botany Department, Lucknow University, Lucknow-226 007 (U.P.), India

*Corresponding author: E-mail, evaaro{at}utu.fi; Fax, +358-2-333 5549.


  Abstract

The protein complexes of pea (Pisum sativum L.) etioplasts, etio-chloroplasts and chloroplasts were examined using 2D Blue Native/SDS–PAGE. The most prominent protein complexes in etioplasts were the ATPase and the Clp and FtsH protease complexes which probably have a crucial role in the biogenesis of etioplasts and chloroplasts. Also the cytochrome b6f (Cyt b6f) complex was assembled in the etioplast membrane, as well as Rubisco, at least partially, in the stroma. These complexes are composed of proteins encoded by both the plastid and nuclear genomes, indicating that a functional cross-talk exists between pea etioplasts and the nucleus. In contrast, the proteins and protein complexes that bind chlorophyll, with the PetD subunit and the entire Cyt b6f complex as an exception, did not accumulate in etioplasts. Nevertheless, some PSII core components such as PsbE and the luminal oxygen-evolvong complex (OEC) proteins PsbO and PsbP accumulated efficiently in etioplasts. After 6 h de-etiolation, a complete PSII core complex appeared with 40% of the maximal photochemical efficiency, but a fully functional PSII was recorded only after 24 h illumination. Similarly, the core complex of PSI was assembled after 6 h illumination, whereas the PSI–light-harvesting complex I was stably assembled only in chloroplasts illuminated for 24 h. Moreover, a battery of proteins responsible for defense against oxidative stress accumulated particularly in etioplasts, including the stromal and thylakoidal forms of ascorbate peroxidase, glutathione reductase and PsbS.

Keywords: Chloroplast - Clp - Etioplast - FtsH - Pea - Protein complex assembly

Abbreviations: APX, ascorbate peroxidase; ATPase, ATP synthase complex; BN, Blue Native; FNR, ferredoxin-NADPH oxidoreductase; GR, glutathione reductase; Fv/Fmax, ratio of variable fluorescence over maximum fluorescence; LHCI, light-harvesting chlorophyll–protein complex I; LHCII, light-harvesting chlorophyll–protein complex II; MS/MS, tandem mass spectroscopy; NDH-1, NAD(P)H dehydrogenase; OEC, oxygen-evolving complex; Pchlide, protochlorophyllide; POR, NADPH-protochlorophyllide oxidoreductase.

(Received November 27, 2007; Accepted January 28, 2008)
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